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Host‐parasite Interaction Model Using Bead‐Grown Biofilms
Author(s) -
Abu-Hassan Diala Walid,
Alkawareek Mahmoud Yousef,
Hamed Rania “Ahmad Azzam”,
Banat Fahmy,
Al-Muhtaseb Saja
Publication year - 2020
Publication title -
the faseb journal
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.709
H-Index - 277
eISSN - 1530-6860
pISSN - 0892-6638
DOI - 10.1096/fasebj.2020.34.s1.09305
Subject(s) - streptococcus oralis , biofilm , microbiology and biotechnology , pseudomonas aeruginosa , christian ministry , bacteria , biology , microorganism , escherichia coli , agar , biochemistry , philosophy , theology , gene , genetics
Infectious diseases are widely spread and can significantly affect people’s lives and cause social and economic damages. Co‐culture systems have been used to investigate different types of cell‐cell interactions just like those that occur in infections between microorganisms and host cells. These techniques have many applications in biology and medicine. Although various co‐culture models using different approaches were published, more modelling strategies are being tested. In this project we present a new co‐culture model based on using agar/glass beads to grow biofilms of different types of bacteria which then can be used to infect cultures of human cells. Biofilms of Staphylococcus aureus, Pseudomonas aeruginosa, Escherichia coli, Streptococcus intermedius , and Streptococcus oralis were successfully grown using this model. Beads containing bacterial biofilms were co‐cultured with PANC1 epithelial cell line to simulate its infection. This co‐culture model proved to be a facile method to study bacteria‐human cell interaction which also can be used to evaluate the efficacy and toxicity of different drug treatments. Support or Funding Information Scientific Research Support Fund, Ministry of Higher Education and Scientific Research, Al Jubaiha, Amman, Jordan 11941.