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Effect of enhancement of hepatic metabolic and glucose homeostasis by gut microbiome regulation of DP in obese mice
Author(s) -
Lee Jung-Eun,
Jung Jeeyoun
Publication year - 2020
Publication title -
the faseb journal
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.709
H-Index - 277
eISSN - 1530-6860
pISSN - 0892-6638
DOI - 10.1096/fasebj.2020.34.s1.09138
Subject(s) - insulin resistance , glucose homeostasis , fatty liver , microbiome , lipidomics , homeostasis , gut flora , medicine , inflammation , endocrinology , obesity , diabetes mellitus , disease , biology , immunology , bioinformatics
The traditional medicine DP is generally used to treat obesity‐related disease and plays central role in the improvement of high‐fat and high‐cholesterol‐induced obesity. In order to reveal the systematic mechanisms of DP on obesity‐related disease, we induced the HFHC diet for 12 weeks in C57BL/6 mice and administrated DP for 6 weeks. After that, we performed non‐targeted lipidomics analysis using mass spectrometry and integrated analysis with various clinical marker of liver. The DP prescription altered liver inflammation status and ameliorated insulin resistance (IR) by regulating GLP‐1 secretion. Additionally, the DP alleviated non‐alcoholic fatty liver disease (NAFLD) through reduce of CD1d‐restricted lipid antigen coupled with γδ‐T cells. More importantly, we have found using microbiome analysis that these mechanisms were regulated by gut microbiota which has been changed by DP treatment. Comprehensively, DP is considered to be an effective herbal decoctions for obesity‐induced systemic hepatic inflammation, glucose homeostasis, and hepatic metabolic homeostasis by regulating microbiome. Therefore, due to the potential therapeutic effects of DP, it is expected to be widely used for the treatment of NAFLD in Korean and Chinese medicine.