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Identifying CoADR and Npsr Function: A Metagenomic Analysis of The Sulfur Reducing Capabilities of CoADR and Npsr
Author(s) -
Wragan Max,
Crane E.J.,
Cavalcanti Andre,
Spindler Archie
Publication year - 2020
Publication title -
the faseb journal
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.709
H-Index - 277
eISSN - 1530-6860
pISSN - 0892-6638
DOI - 10.1096/fasebj.2020.34.s1.08867
Subject(s) - metagenomics , biology , biochemistry , gene
Coenzyme‐A disulfide reductase (CoADR) and NADH‐dependent persulfide reductase (Npsr) are enzymes that have long been suspected to metabolize inorganic sulfur compounds due to their anaerobic environments in which they are found. Previous studies have had difficulty proving that CoADR and Npsr perform sulfur respiration through direct approach, which is why this research explored CoADR and Npsr’s relationship with sulfur reduction indirectly through metagenomic analysis. These results compiled the geochemistry, biochemistry, and microbiology of a wide variety of proteins with known functions and explored their relationship with CoADR and Npsr. Our data was compiled through the Joint Genome Institute’s metagenome database and run utilizing their BLAST program. This data was analyzed through Principle Component Analysis (PCA) which suggested CoADR and Npsr’s role as sulfur reducing enzymes via their correlation and alignment with other known sulfur reducing proteins, and their lack of their of with sulfate reducing, oxidative stress, and reversible sulfur cycling proteins. Support or Funding Information Funding for research came from NSF grant to Dr. EJ Crane. Research was conducted at the Department of Molecular Biology, Pomona College, Claremont, CAContributors: Max Wragan, Archie Spindler, Dr. EJ Crane, and Dr. Andre CavalcantiPlots of the scores of each environment in the first two principal components. Aerobic environments are shown in teal and anaerobic environments are shown in orange. The 95% confidence ellipses are also shown for each category.Variable PCA with grouping. Each arrow represents a protein. The color of each arrow corresponds to the predicted function of the protein. Dimensions 2 and 3 were utilized, rather than the default Dimensions 1 and 2, to increase clarity of figure. CoADR and Npsr were found to group among known Elemental Sulfur Reducing Proteins—PsrA, SudHa, and SudHb—suggesting CoADR and Npsr also act as elemental sulfur reducers