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CDC20 and TRIB3 Upregulated by TFAP2C Facilitate Cell Proliferation in Non‐small Cell Lung Cancer Cells
Author(s) -
Kim Dain,
Seo Danbi,
Kim Wanyeon
Publication year - 2020
Publication title -
the faseb journal
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.709
H-Index - 277
eISSN - 1530-6860
pISSN - 0892-6638
DOI - 10.1096/fasebj.2020.34.s1.07623
Subject(s) - downregulation and upregulation , cancer research , cell growth , microarray analysis techniques , carcinogenesis , biology , cell cycle , gene knockdown , transcription factor , skp2 , cancer , apoptosis , gene expression , ubiquitin ligase , ubiquitin , gene , genetics
In recent years, non‐small cell lung cancer (NSCLC) is the most serious cause of global cancer‐related mortality. To resolve this circumstance, many studies for identification of novel molecular targets have been conducted. In our previous investigation, a microarray analysis that associated with transcription factor activating enhancer‐binding protein 2C (TFAP2C) was carried out and we found out TFAP2C's oncogenic roles in NSCLC development. In the current study, we intended to reveal novel biomarkers for NSCLC, especially focused on several oncogenes transcriptionally regulated by TFAP2C. With the result of microarray analysis, cell division cycle 20 (CDC20) and tribbles pseudokinase 3 (TRIB3) were selected as promising oncogenes by transcriptionally upregulated by TFAP2C. In the treatment of TFAP2C‐specific siRNA in two NSCLC cell lines (NCI‐H292 and NCI‐H838 cells), we observed that mRNA and protein levels of CDC20 and TRIB3 were down‐regulated. In addition, when CDC20 or TRIB3 was overexpressed, cell viability was increased and reversely apoptosis of these cells was suppressed. Consequently, these results indicated that NSCLC tumorigenesis affected by two promising oncogenes, CDC20 and TRIB3, and they could act as potent markers to prognosis of NSCLC. Support or Funding Information This work was supported by the National Research Foundation of Korea (NRF) funded by the Ministry of Science and ICT (2019R1C1C1009423).