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CAROGENIC MICROORGANISMS IN CHILDREN WITH PRIMARY AND MIXED DENTITION
Author(s) -
Rodriguez Patricia Noemi,
Mateo Maria Teresa,
Iglesias Maria Veronica,
Alonso Maria Gabriela,
Lifshitz Fima,
Molgatini Susana,
Friedman Silvia Maria,
Canga Liliana
Publication year - 2020
Publication title -
the faseb journal
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.709
H-Index - 277
eISSN - 1530-6860
pISSN - 0892-6638
DOI - 10.1096/fasebj.2020.34.s1.07501
Subject(s) - veillonella , biofilm , microorganism , microbiology and biotechnology , dentistry , agar , biology , food science , chemistry , medicine , streptococcus , bacteria , genetics
The oral cavity is one of the most important and complex habitats of microorganisms, housing a great diversity of communities, which colonize the mucous membranes and form the dental biofilm. The acidification of biofilm by carbohydrate intake is an important factor for the onset and progression of carious lesions, where the microorganisms involved produce acids and tolerate a low pH medium. Objective Determine in children with and without carious lesions the composition of the supragingival biofilm according to the type of dentition. Materials and methods The study population included 49 children (24 with primary dentition PD, 36–72 months of age and 25 with mixed dentition MD, 74 to 110 months of age), with and without carious lesions. Presence of caries was determined by the visual method ICDAS and dietary history was recorded. To perform microbiological study of acidogenic microorganisms (SM: Streptococcus spp , L: Lactobacillus spp , C: Candida spp . and V: Veillonella spp ), a sample of supragingival biofilm plaque was collected from molar. Agar mitis salivarius with bacitracin, MRS agar (Britany®Argentina), selective and differential medium CHROMagar Candida and selective medium for Veillonella with the addition of vancomycin and lacquered blood were inoculated. All were incubated under atmospheric conditions according to the requirements of each of the studied microorganisms. Results were statistically analyzed using ANOVA or Fisher, when appropriate. The study was approved by the Ethics Committee of the School of Dentistry‐UBA. Results SM and L counts was higher in PD, regardless of the presence of caries, p< 0.0001. In PD the percentage of positive C cultures was higher in children with caries, differences that were not observed in MD (p=0.0478). With respect to V, only a higher percentage of positive crops were observed in children with caries in MD (p=0.0119). Conclusion the count of SM and L and the presence of C and V vary with the type of dentition, suggesting a change in the composition of the microbiota, even in teeth without caries. Support or Funding Information Granted by UBACYT 20720170100005BA.