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The Dynamic Proteome and Lipidome of Plastoglobule Lipid Droplets of Plant Plastids
Author(s) -
Lundquist Peter Knut,
Espinoza-Corral Roberto
Publication year - 2020
Publication title -
the faseb journal
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.709
H-Index - 277
eISSN - 1530-6860
pISSN - 0892-6638
DOI - 10.1096/fasebj.2020.34.s1.07364
Subject(s) - lipidome , proteome , lipid droplet , plastid , lipidomics , arabidopsis thaliana , arabidopsis , microbiology and biotechnology , chemistry , lipid bilayer , biology , biochemistry , biophysics , botany , membrane , chloroplast , gene , mutant
Plastoglobules are ubiquitous lipid droplets of plant plastids that consist of a lipidic core that is enclosed by a monolayer membrane surface studded with structural and enzymatic protein. The dramatic changes in plastoglobule morphology in response to environmental stresses, including more than 10‐fold increases in droplet diameter, suggest a crucial role in plant stress tolerance. However, in the absence of an understanding of protein or lipid changes of the plastoglobule under stress, it is difficult to connect the plastoglobule to specific functions. We have used proteomic and lipidomic profiling to determine the quantitative protein and lipid compositions, and oligomeric state of isolated plastoglobules from Arabidopsis thaliana under unstressed and high light treatment conditions. Our analysis has revealed evidence of multiple high molecular weight complexes at the plastoglobule and changes to the prenyl‐lipid composition. Building on our results, we propose a model of plastoglobule function related to mediating membrane remodeling processes in response to stress adaptation. Support or Funding Information Michigan State University, MSU AgBioResearch