Mo lecular Tr ansducers of P hysical A ctivity C onsortium (MoTrPAC) Progress

Physical activity is beneficial to human health and well being across the lifespan. The numerous benefits of regular physical activity have long been recognized. Despite this, most exercise studies are associational and the molecular mechanisms that are the bases for the beneficial effects remain obscure as are the mechanisms of multi organ communications and benefits. The NIH Common Fund initiated the Molecular Transducers of Physical Activity Consortium (MoTrPAC) in December 2016 by issuing 19 grants to 37 Principal Investigators from 23 institutions. Fundamentally, MoTrPAC is a large discovery project the goals of which are to: Aim 1: Assemble a comprehensive map of the molecular changes that occur in response to exercise and provide insights into how they are altered by age, sex, body composition and fitness level. Aim 2: Develop a user‐friendly database to facilitate investigator‐initiated studies and catalyze the field of physical activity research whereby researchers can develop hypotheses exploring novel mechanisms by which physical activity improves or preserves health. This project will explore and document changes in molecules mobilized in blood, muscle and fat in humans as well as 15 additional tissues for rats in response to different exercise regimes. The human studies are a multi‐center clinical trial cohort of people of both sexes from 10–80 years of age and recruitment has begun. Preclinical Animal Study Sites (PASS) have conducted treadmill running experiments in male and female 6 and 18‐month old F344 rats and harvested tissues from control inactive rats and rats at seven time‐points following a single 30 minute acute bout of treadmill running (70% VO2max) in their active phase (dark). A separate group of rats were subjected to an intensive (also 70% VO2max) and progressive run training program of 5 day/week for 1,2, 4, or 8 weeks, also in their active phase, with 18 tissues collected per rat. Multiple state of art and omics platforms including genomic, transcriptomic, epigenomic, proteomic and metabolomics are being employed to define and discover the molecules mobilized in response to exercise that will serve as a valuable resource for the research community and support future investigator‐initiated studies to understand the molecular mechanisms of exercises effects. PASS tissues not analyzed are stored in the MoTrPAC BioRepository and will be available for ancillary studies. This presentation will summarize data from the initial public data release (November 15 th , 2019; five most abundant tissues). Data will be available to the extramural community to collaborate with the MoTrPAC investigators to explore innovative mechanisms to expand the impact of the MoTrPAC studies. Support or Funding Information NIH Common Fund