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Pontine Parabrachial Nucleus (PBN) Neuron Subtypes Involved With the Control of Breathing Frequency
Author(s) -
Zuperku Edward J.,
Hopp Francis A.,
Stuth Eckehard A.,
Stucke Astrid G.
Publication year - 2020
Publication title -
the faseb journal
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.709
H-Index - 277
eISSN - 1530-6860
pISSN - 0892-6638
DOI - 10.1096/fasebj.2020.34.s1.06407
Subject(s) - bicuculline , neuroscience , ampa receptor , pons , glutamate receptor , lateral parabrachial nucleus , neuron , ventilation (architecture) , medicine , premovement neuronal activity , chemistry , antagonist , parabrachial nucleus , receptor , biology , nucleus , physics , thermodynamics
A subregion within the medial PBN plays a key role in the control of expiratory duration (TE) and breathing frequency (fB) 1. Drug‐induced increases in neuronal activity decrease TE, while depression of these neurons increases TE. Activity from these neurons is relayed with short latencies to neurons in the preBötzinger/Bötzinger region 2. This study analyzed the relationship between charateristics of the discharge frequency (Fn) patterns of I, E, phase spanning and non‐respiratory modulated (NRM) PBN neurons and changes in fB induced by local application of neural transmitter agonist and antagonist. Multichannel neuronal recording with highly localized microinjections into the PBN were performed in decerebrate, vagotomized dogs during isocapnic hyperoxia. The phrenic neurogram was used to assess fB responses and identify neuronal discharge patterns. Plots of fB vs. neuronal Fn during induced fB responses produced by microinjections of AMPA and the GABA‐A receptor antagonist bicuculline (BIC) were linear with high correlation values (r > 0.75). Furthermore, it appears that neurons with little or no respiratory modulation appear to be more highly correlated with fB than neurons with strong phasic patterns, suggesting that these neurons may be responsible for the pontine control of fB. Support or Funding Information Supported by Department of Veterans Affairs grant 2 I01BX000721‐08 (Zuperku) & R01GM112960‐01A1 (Stucke)Example of breathing frequency response (BPM) to microinjected AMPA (green) in PBN and corresponding neuronal responses (red) for 2 neurons (panel A and panel B). Lower: Plots of BPM vs discharge frequency (Fn = Fmax) for two simultaneously recorded neurons, phasic‐I (A) and NRM (B) with corresponding slope and r‐squared values. Top: Cycle‐Triggered Histograms (CTHs)1 Zuperku , E.J. , Stucke , A.G. , Hopp , F.A. , Stuth , E.A. , 2017 . Characteristics of breathing rate control mediated by a subregion within the pontine parabrachial complex . J. Neurophysiol. 117 , 1030 – 1042 . 2 Zuperku EJ , Stucke AG , Krolikowski JG , Tomlinson J , Hopp FA & Stuth EA. ( 2019 ). Inputs to medullary respiratory neurons from a pontine subregion that controls breathing frequency . Respir Physiol Neurobiol . 265 , 127 – 140 .