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Serum Extracellular Vesicles Derived From Obese Mice Control Energy Expenditure and Promote Liver Steatosis in Lean Mice
Author(s) -
Cunha e Rocha Karina,
de Mendonça Mariana,
Rodrigues Alice Cristina
Publication year - 2020
Publication title -
the faseb journal
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.709
H-Index - 277
eISSN - 1530-6860
pISSN - 0892-6638
DOI - 10.1096/fasebj.2020.34.s1.06307
Subject(s) - endocrinology , medicine , steatosis , fatty liver , triglyceride , obesity , respiratory exchange ratio , insulin resistance , cholesterol , heart rate , disease , blood pressure
Cells can communicate with neighboring or distant cells trough extracellular vesicles (EVs). EVs have been shown to participate in many diseases, however, little is known about their role in metabolic diseases. Obesity is a major risk factor for noncommunicable diseases, and represents a threat to global public health. Indeed, obesity is rising in incidence, prevalence, and economic burden. Once the number of released EVs and its cargo are altered during obesity, we hypothesize that EVs intercellular communication may play a role in obesity pathophysiology. In order to investigate the metabolic changes caused by EVs in obesity, C57BL/6 male mice fed a control balanced diet were injected (i.p.) with serum EVs derived from diet‐induced obese mice (400ug/mouse) every 3 days for 8 weeks. Body weight was assessed every week. Visceral and subcutaneous fat depots, liver and gastrocnemius and soleus muscles were dissected and weighed. Serum biochemical analysis were conducted, and the content of hepatic triglyceride and total cholesterol was measured. Glucose, insulin and pyruvate tolerance tests were performed, as well as body composition analysis and indirect calorimetry. Differences at p<0.05 were considered significant. Compared to the control group, EVs injection attenuated weight gain, reduced the weight of subcutaneous fat depot, gastrocnemius muscle and liver, decreased the respiratory exchange ratio and increased hepatic triglyceride content, oxygen consumption and insulin sensitivity. Our results suggest that EVs released in obesity can modulate lean mice metabolism. Altogether, these data provide initial insights on EVs function and signaling during obesity. Support or Funding Information This study was financed in part by grants #2018/05426‐0 and #2019/14999‐6, São Paulo Research Foundation (FAPESP) and the Coordenação de Aperfeiçoamento de Pessoal de Nível Superior ‐ Brasil (CAPES) ‐ Finance Code 001.