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Store‐Operated Calcium Entry Regulates Macrophage Chemotaxis
Author(s) -
White Carl,
Fresquez Adriana M.
Publication year - 2020
Publication title -
the faseb journal
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.709
H-Index - 277
eISSN - 1530-6860
pISSN - 0892-6638
DOI - 10.1096/fasebj.2020.34.s1.06014
Subject(s) - chemotaxis , microbiology and biotechnology , macrophage , inflammation , calcium , chemistry , biology , immunology , in vitro , receptor , biochemistry , organic chemistry
The store operated calcium entry (SOCE) pathway is known to be important for cell migration; however, its involvement in regulating directed movement toward a chemotactic signal is less well understood. The aim of current study was to define the involvement of SOCE in macrophage chemotaxis. To achieve this aim, we used phase contrast microscopy to visualize primary mouse bone marrow derived macrophages migrating toward the source of the potent chemoattractant C5a (complement component 5a). A concentration gradient was established by pulsing C5a from a micropipette connected to a pressure regulating device. Chemotaxis was monitored for up to 90 minutes in the presence and absence of the SOCE blocker BTP2 (1 μM). We found that the chemotactic speed and total distance traveled was decreased in the presence BTP2. In addition, we quantified the morphological changes at the leading edge of the migrating cells and found that frequency and speed of protrusions was also reduced by BTP2. These studies establish the requirement for SOCE in macrophage chemotaxis and leading‐edge dynamics and suggest a role for SOCE in regulating the recruitment of macrophages to sites of inflammation in vivo. Support or Funding Information This work was supported by NIH Grant HL142906 to C.W.