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Exploring the Effect of Growth Conditions on the Volatile Metabolome of Chromobacterium vaccinii using GC×GC‐TOF‐MS
Author(s) -
Ramirez Diana,
Higgins Keppler Emily A.,
Bean Heather D.
Publication year - 2020
Publication title -
the faseb journal
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.709
H-Index - 277
eISSN - 1530-6860
pISSN - 0892-6638
DOI - 10.1096/fasebj.2020.34.s1.05711
Subject(s) - metabolome , petri dish , chromobacterium violaceum , chemistry , agar , metabolomics , chromatography , food science , bacteria , biology , microbiology and biotechnology , biochemistry , quorum sensing , genetics , virulence , gene
Chromobacterium spp. have been found to grow in a variety of environments and have an extensive metabolic flexibility. Due to this flexibility, studies have shown that Chromobacterium spp. produce an array of properties that can positively impact medicine, industry, and agriculture. C. vaccinii , isolated from cranberry bog soil, has been found to inhibit the growth of multiple pathogenic plant fungi through the production of volatile compounds. Fungal inhibition has been observed when the fungi is co‐cultured with C. vaccinii in separate petri dishes but with shared gas exchange. The same fungal inhibition is not observed when the fungus is exposed to C. vaccinii metabolites produced in liquid media. Based on this observation, we hypothesize that the volatiles produced in liquid media are not the same as the volatiles produced in agar. Experiments are being conducted to study the influence of growth conditions on C. vaccinii volatile metabolites. C. vaccinii will be cultured in liquid and semi‐solid media, the volatiles will be collected using thin film solid phase microextraction (TF‐SPME) and analyzed with two‐dimensional gas chromatography – time‐of‐flight mass spectroscopy (GC×GC‐TOFMS). The goal is to determine the differences between the volatiles produced in the different types of media. These results will lead to a better understanding of this natural occurring soil bacteria. Support or Funding Information NSF LEAP Scholars Program