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The Effect of Different Magnesium Sources on Renal Magnesium Transporters in Rats Fed a High Fat Diet
Author(s) -
Sahin Kazim,
Yazgan Kubra,
Tuzcu Mehmet,
Orhan Cemal,
Sahin Nurhan,
Ojalvo Sara Perez,
Sylla Sarah,
Komorowski James R.
Publication year - 2020
Publication title -
the faseb journal
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.709
H-Index - 277
eISSN - 1530-6860
pISSN - 0892-6638
DOI - 10.1096/fasebj.2020.34.s1.05627
Subject(s) - hypomagnesemia , medicine , endocrinology , magnesium , creatinine , chemistry , kidney , urea , biochemistry , organic chemistry
Magnesium (Mg), which plays a role in many biochemical functions of the body, is the second most common cation in the cell. A high‐fat diet (HFD) intake causes hypomagnesemia, which is an electrolyte disorder. In this study, the molecular effects of Mg forms [magnesium oxide (MgO); magnesium picolinate (MgPic)] on renal Mg transporters (TRPM6, TRPM7, EGF, claudin‐16, claudin‐19, NCC) were investigated in HFD rats. For this purpose, 42 male Sprague‐Dawley (8 weeks old) rats were divided into 6 groups 1) Control; 2) MgO; 3) MgPic; 4) HFD; 5) HFD+MgO; 6) HFD+MgPic. Mg was supplemented at 500 mg of elemental Mg/kg diet. No effect on serum creatinine and urea levels of both Mg forms was detected ( P >0.05). In HFD rats, kidney Mg levels were lower and kidney MDA levels were higher than other groups. However, the kidney Mg levels were increased and the kidney MDA levels were decreased by the Mg supplementation ( P <0.001). The highest increase/decrease was obtained in the MgPic group. In addition, TRPM6, TRPM7, NCC, EGF, claudin16 and claudin19 Mg transporters were negatively affected by the HFD intake. However, it has been determined that Mg forms, especially MgPic, improved the levels of these proteins ( P <0.001). As a result, MgPic may prevent oxidative stress and regulate Mg transporters in kidney including TRPM6, TRPM7, NCC, claudin16, and claudin19 in HFD rats Support or Funding Information Nutrition21, NY Firat Universitry ‐VF.18.02Turkish Academy of Science (KS)