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Breathing Pattern Differs Between Male and Female Rats in Escherichia coli Induced Septicemia
Author(s) -
Campanaro Cara,
Clifford Caitlyn,
Tackett Shiloh,
Horton Kofi-Kermit,
Nethery David,
Hsieh Yee-Hsee,
Dick Thomas,
Jacono Frank
Publication year - 2020
Publication title -
the faseb journal
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.709
H-Index - 277
eISSN - 1530-6860
pISSN - 0892-6638
DOI - 10.1096/fasebj.2020.34.s1.05565
Subject(s) - escherichia coli , plethysmograph , inoculation , biology , medicine , respiratory rate , zoology , endocrinology , immunology , heart rate , biochemistry , gene , blood pressure
Septicemia is a potentially fatal infection marked by bacteria in the bloodstream coupled with a dysregulated inflammatory response. Escherichia coli ( E. coli ) is a common cause of septicemia. We hypothesized that in E. coli induced septicemia, respiratory frequency (fR) and the ventilatory pattern variability (VPV) would depend on sex. Male (n=6) and female (n=6) Sprague Dawley rats were inoculated with E. coli (100×10 6 colony forming units) contained within a clot formed of thrombin, fibrinogen, and phosphate buffered saline. We measured ventilation in unanesthetized rats with whole‐body plethysmography before (0h) and 12h after inoculation. Before inoculation, the fR male rats was greater than that of females (fR: 78.4 ± 11.9 vs. 58.7±6.98 breaths per minute (brthspm); p=0.007). In contrast, VPV did not differ between males and female (autocorrelation coefficient (r) at 1 cycle length 0.58±0.17 vs 0.56±0.13 and sample entropy 1.24±0.04 vs 1.34±0.11 Nats). However, 12h after inoculation, fR in male rats was much greater than female (142.73±22.03 vs. 64.59±6.61 brthspm; p= 0.0001) and analysis of VPV found differences in autocorrelation (0.814±0.084 vs. 0.578±0.160; p=0.01) and sample entropy (0.98±0.10 vs. 1.19±0.13 Nats; p=0.01). Further, in both male and female rats, fR increased 12h after inoculation rats (males, p=0.001; females p=0.02). These preliminary data indicate that male rats have a higher resting fR and greater ventilatory response to septicemia than female rats. Pending analysis of pro‐inflammatory cytokines will indicate whether the magnitude of these ventilatory response correlates to the magnitude of the inflammatory response. Support or Funding Information Funded by: VA Research Service (I01BX004197); NIH (U01 EB021960)