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Molecular and structural characterization of a TEAD mutation at the origin of Sveinsson’s chorioretinal atrophy
Author(s) -
Bokhovchuk Fedir,
Mesrouze Yannick,
Izaac Aude,
Meyerhofer Marco,
Zimmermann Catherine,
Fontana Patrizia,
Schmelzle Tobias,
Erdmann Dirk,
Furet Pascal,
Kallen Joerg,
Chène Patrick
Publication year - 2020
Publication title -
the faseb journal
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.709
H-Index - 277
eISSN - 1530-6860
pISSN - 0892-6638
DOI - 10.1096/fasebj.2020.34.s1.05546
Subject(s) - mutant , mutation , genetics , hippo signaling pathway , biology , gene , transcription factor , microbiology and biotechnology , chemistry
Four TEAD transcription factors (TEAD1–4) mediate the signalling output of the Hippo pathway that controls organ size in humans. TEAD transcriptional activity is regulated via interactions with the YAP, TAZ and VGLL proteins. A mutation in the TEAD1 gene, Tyr421His, has been identified in patients suffering from Sveinsson’s chorioretinal atrophy (SCA), an autosomal dominant eye disease. This mutation prevents the YAP/TAZ:TEAD1 interaction. In this study, we measure the affinity of YAP, TAZ and VGLL1 for the four human TEADs and find that they have a similar affinity for all TEADs. We quantitate the effect of the mutation found in SCA patients and show that it destabilizes the YAP/TAZ:TEAD interaction by about 3 kcal·mol–1. We determine the structure of YAP in complex with this mutant form of TEAD and show that the histidine residue adopts different conformations at the binding interface. The presence of this flexible residue induces the destabilization of several H‐bonds and the loss of van der Waals contacts, which explains why the Tyr421HisTEAD1 mutation has such a large destabilizing effect on the formation of the YAP:TEAD complex.Close view of the region surrounding key Tyr/His429 in the superimosed wtYAP:wtTEAD4 and wtYAP:Tyr429HisTEAD4 complexes