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The effect of Ananixanthone in TGF‐ ß1 signaling
Author(s) -
Yeh Yu-Hsuan,
Chen Jih-Jung,
Chen Chun-Lin
Publication year - 2020
Publication title -
the faseb journal
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.709
H-Index - 277
eISSN - 1530-6860
pISSN - 0892-6638
DOI - 10.1096/fasebj.2020.34.s1.05366
Subject(s) - xanthone , transforming growth factor , epithelial–mesenchymal transition , fibronectin , chemistry , signal transduction , cancer research , phosphorylation , transforming growth factor beta , cadherin , cell growth , microbiology and biotechnology , cell , transition (genetics) , biology , gene , biochemistry , stereochemistry
Transforming growth factor beta 1(TGF‐ß1) is a famous known growth factor which promotes malignancy and enhances tumor metastasis by inducing epithelial‐mesenchymal transition (EMT). Ananixanthone(GMP15), a derivative of xanthone, is extracted from Calophyllum teysmannii and has been reported to show lower toxicity compared to xanthone. Previous study has showed that xanthone repressed TGF‐ ß1‐mediated EMT, however there is no study about GMP15. Hence, in this study, we determine to study the effect of GMP15 in TGF‐ ß1 signaling. We found that GMP15 suppressed TGF‐β1‐induced PAI‐1 reporter gene activation and Smad2/3 phosphorylation in various type of cell. Also, GMP15 repressed TGF‐ ß1‐mediated expression of N‐cadherin and Fibronectin as well as abolished repression of E‐cadherin in HepG2 cell, thus blocking TGF‐ ß1‐mediated EMT. Our preliminary finding showed that GMP15 might be a potential inhibitor of TGF‐ ß1 signaling. Finding the underlying mechanism of GMP15 in TGF‐ ß1 signaling might help provide a new choice of cancer treatment.