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Efficacy of the curcumin to ameliorate ovalbumin induced atopic dermatitis and progression of atopic march in mice
Author(s) -
SHARMA SUKRITI,
NAURA AMARJIT SINGH
Publication year - 2020
Publication title -
the faseb journal
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.709
H-Index - 277
eISSN - 1530-6860
pISSN - 0892-6638
DOI - 10.1096/fasebj.2020.34.s1.05092
Subject(s) - curcumin , atopic dermatitis , ovalbumin , medicine , sensitization , pharmacology , proinflammatory cytokine , immunology , oxidative stress , immune system , inflammation
Atopic dermatitis (AD) is a chronic pruritic skin inflammatory condition which arises due to skin barrier disruption and immune dysregulation. Curcumin has been widely exploited in the treatment of various skin‐related pathologies due to its pharmacologic effects. The present study was designed to investigate the beneficial effect of curcumin in the amelioration of ovalbumin (OVA)‐induced atopic dermatitis and ensuing atopic march. AD was induced in female Balb/c mice by epicutaneous application of OVA at the back skin site for 1 week followed by a resting period of two‐weeks and same protocol was repeated thrice. Curcumin treatment was given either intraperitoneally or topically in two separate groups of animals. Curcumin was administered intraperitoneally at different doses during the last sensitization phase for a period of 7 days. Topical application of curcumin at a dose of 10 mg/kg was done for a period of 14 days during the second resting period. Skin tissues were examined for histologic changes, Th2 cytokines and the oxidative stress markers. Systemic curcumin administration ameliorated the onset of AD‐like lesions and significantly improved the dermal destruction in mice upon OVA exposure. Curcumin reduced the levels of various inflammatory mediators such as Th2 promoting cytokines (TSLP and IL‐33), Th2 cytokines (IL‐4, IL‐5, IL‐13 and IL‐31) and also downregulated the activation/expression of Stat‐6 and GATA‐3. Next, when curcumin preparation was applied topically, it shows a remarkable efficacy in restoration of the histo‐architecture of the skin suggesting that the phytochemical based ointment(s) can offer a newer treatment option for AD and associated atopic march. Finally, curcumin blocked the OVA initiated progression of AD towards asthma as evident by the reduced number of BALF inflammatory cells and suppressed expression of Th2 cytokines in the lung tissue upon aerosolized exposure of the allergen to the AD mice. Overall, our findings demonstrate for the first time that curcumin has the efficacy to ameliorate AD and halt the progression of associated atopic march. Support or Funding Information Financial support from Department of Biotechnology, Government of India [BT/PR17968/MED/122/33/2016]; University Grants Commission, New Delhi, India [UGC‐SAP] and [UGC‐BSR‐SRF]