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In Vitro Rat intestinal α‐glucosidase inhibitory and Antioxidant Activities of Phenolic Compounds isolated from Colored Rice Keun‐nun‐Jami ( Oryza sativa L .) Germ Extracts
Author(s) -
Kim Tae-Yang,
Kang Hanna,
Yu Jin-a,
Mitiku Haimanot,
Hong Sung-Gil,
Ryu Su-Noh,
Kwon Young-In
Publication year - 2020
Publication title -
the faseb journal
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.709
H-Index - 277
eISSN - 1530-6860
pISSN - 0892-6638
DOI - 10.1096/fasebj.2020.34.s1.04937
Subject(s) - postprandial , oxygen radical absorbance capacity , sucrase , antioxidant , alpha glucosidase , chemistry , acarbose , carbohydrate , biochemistry , maltase , digestion (alchemy) , food science , functional food , ethyl acetate , polyphenol , flavanone , enzyme , dpph , chromatography , diabetes mellitus , biology , endocrinology , flavonoid
Excessive carbohydrate‐based meals may result in postprandial hyperglycemia of type 2 diabetes patients, a modern human metabolic disorder. Therefore Inhibition of α‐glucosidase involved in the digestion and absorption of carbohydrates can significantly decrease the postprandial increase of blood glucose level after a carbohydrate diet. In the screening of anti‐hyperglycemic and antioxidant compounds from the extract of Keun‐nun‐jami Germ (KGE), several bioactive ingredients were isolated and identified from ethyl acetate extraction of KGE using HPLC and MPLC systems. Their structures were elucidated by extensive spectroscopic analysis, including 1D and 2D NMR, and FTICR‐MS. To prove the anti‐hyperglycemic effect of purified compound A and B, inhibitory activities of these compounds against rat intestinal α‐glucosidases (sucrase) were investigated. Furthermore, antioxidant activities of these compounds were also evaluated by oxygen radical absorbance capacity (ORAC) method. A major bioactive compound A in KGE had high rat intestinal sucrase inhibitory activity as well as antioxidant activity. Although we need to identify full structure analysis of these compounds and in‐vivo assay, a major bioactive compound A which has high antioxidant and anti‐hyperglycemic activities has the potential to contribute as a useful food supplements for controlling postprandial hyperglycemia.