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SPARC: Topographical Organization and Morphology of Substance P‐IR Axons and Terminals in the Whole‐Mount Stomach of Rodents
Author(s) -
Ma Jichao Mark,
Chen Jin,
Powley Terry L.,
Cheng Zixi Jack
Publication year - 2020
Publication title -
the faseb journal
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.709
H-Index - 277
eISSN - 1530-6860
pISSN - 0892-6638
DOI - 10.1096/fasebj.2020.34.s1.04918
Subject(s) - antrum , stomach , anatomy , myenteric plexus , fundus (uterus) , substance p , dorsum , axon , afferent , biology , nociception , neuroscience , immunohistochemistry , medicine , pathology , receptor , neuropeptide , biochemistry , ophthalmology
Afferent fibers innervate the stomach. However, very little is known about the topographical distribution and morphological terminal structures of gastric nociceptive axons and terminals in rodents. In this study, we used Substance P (SP) as a marker to label afferent fibers in the whole‐mount dorsal and ventral stomach including longitudinal and circular muscles and the myenteric nervous system in C57BL/6J mice and SD rats. The whole‐mount dorsal and ventral stomachs were processed with the substance P (SP) primary antibody followed by the secondary antibody, and then examined using confocal microscopy. We found that: 1) SP‐IR axons and terminals extensively covered the fundus, corpus and antrum of the dorsal and ventral stomach. 2) SP‐IR varicose axons ran in parallel with the longitudinal as well as circular muscles. 3) SP‐IR varicose axons formed dense rings of terminal varicosities around individual neurons in the myenteric plexus. 4) The density of SP‐IR axons and terminals varicosities was different in the fundus, corpus and antrum. Collectively, our data showed for the first time the comprehensive topographical distribution and morphology of SP‐IR axons and terminals in different regions of the stomach. This knowledge will provide an anatomical basis for differentiated nociceptive gastric afferent functions in rodents. Support or Funding Information NIH HEAL/SPARC Program NIH 1 U01 NS113867‐01 and NIH R15 HL137143‐01A1.