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CTRP3 and Alcoholic Liver Disease in Female Mice
Author(s) -
Root Callie Leah
Publication year - 2020
Publication title -
the faseb journal
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.709
H-Index - 277
eISSN - 1530-6860
pISSN - 0892-6638
DOI - 10.1096/fasebj.2020.34.s1.04346
Subject(s) - steatosis , medicine , endocrinology , triglyceride , adipokine , adipose tissue , analysis of variance , confounding , alcoholic liver disease , fatty liver , ethanol , chemistry , biology , obesity , leptin , cholesterol , disease , biochemistry , cirrhosis
C1q TNF Related Protein 3 (CTRP3), is a cytokine that is primarily secreted from adipose tissue, also known as an adipokine. Our previous research has shown that CTRP3 prevents alcoholic fatty liver disease (ALD) in male mice. However, even when accounting for confounding factors such as absolute and relative alcohol intake females are more sensitive to the effects of ethanol compared to male mice. Therefore, the goal of this project was to determine whether CTRP3 prevented ALD in female mice or determine whether the beneficial effects of CTRP3 are sex specific. Methods Female wild type (WT) and CTRP3 transgenic over expressing (Tg) mice were fed an ethanol containing liquid diet (5% v/v) for 6 weeks. Daily weight and food intake measurements were taken and external heat‐pads were placed under a portion of the cage to facilite thermoregulation. Hepatic steatosis was determined by total triglyceride quantification and lipid droplet quantitation in liver sections. Data were analyzed by repeated measures ANOVA, t‐test, or Log‐rank (Mantel‐Cox) test as appropriate. Results There was no difference between WT and Tg mice in food intake or body weight. There was no difference in survival between WT and Tg mice, however, Tg mice trended towards a reduced rate of survival compared with WT mice (78% in WT versus 44% in Tg, p=0.13). Stereological analysis indicated no difference in the percent of lipid liver volume between the two groups (WT 7.2±3.6 vs Tg 5.1±4.1%). This finding was consistent with no difference in total hepatic triglyceride accumulation observed between WT and Tg mice (12.7±4.4 vs. 13.1±6.8 mg triglycerides/gram liver protein). Conclusion Combined these data indicate that unlike previous studies with male mice, CTRP3 is not protective against alcohol‐induced hepatic steatosis. Combined, these data indicate that the adipokines such as CTRP3 contribute to physiology in a sex‐specific manner.