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Morphometric Characterization of Extracellular Vesicles in Adults with Obesity
Author(s) -
Alvarado Herminia Mendivil,
Munguia Bibiana Chavez,
Millan Elizabeth Carvajal,
Hernandez Manuel Hernandez,
Teros Veronica Lopez,
Moreno Bertha Pacheco,
Duran Lucila Rascon,
Garcia Humberto Astiazaran
Publication year - 2020
Publication title -
the faseb journal
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.709
H-Index - 277
eISSN - 1530-6860
pISSN - 0892-6638
DOI - 10.1096/fasebj.2020.34.s1.04032
Subject(s) - microvesicles , extracellular vesicles , exosome , extracellular , western blot , obesity , nanoparticle tracking analysis , body mass index , extracellular vesicle , body weight , endocrinology , medicine , andrology , chemistry , biology , physiology , biochemistry , microrna , microbiology and biotechnology , gene
Extracellular vesicles (EVs) are an extracellular communication system. Recently, they have more studied for their function as intercelular communication vehicles and their participation in physiological processes and specific health conditions. However, little is know about the influence of excess weight on their morphometric characteristics. The aim was to characterize populations of extracellular vesicles in adults with obesity and normal weight. Study design was cross‐sectional and subjects were assigned to: group A (n=10) which included participants with obesity ( Body Mass Index (BMI)>30 kg/m 2 ), and group B (n=10) included subjects with normal weight (BMI 18 – <25 kg/m 2 ). Subjects were free of co‐morbidities. Weight, height, electrical bioimpedance, and serum lipids were evaluated. EVs were isolated from blood plasma using Invitrogen total exosome isolation reagent. Isolation was later corroborated by detecting the expression of the marker ALIX, an EV‐associated protein by Western blot (WB), and the particle size was assessed using Zetasizer Nano ZS. The isolated EVs were also analyzed by transmission electron microscopy (TEM). We identified two sizes of EVs, that were later classified as exosomes and microvesicles. The characterization of EVs was confirmed with the expression of ALIX and the images of TEM. In subjects from group A, EVs size (65.9 ± 16.1nm) was larger than in those subjects without obesity (42.3 ± 10.7nm), but this was not statistically significant. Body weight and tryglycerides concentration were positively associated (p<0.05) with EVs’ size. The variability in the size of EVs between groups could reflect different composition or maturation level of EVs. The size of EVs is related with state of obesity, also its content could reflect higher risk of developing comorbidities associated with excess weight and nutritional status. Support or Funding Information The project was funded by CIAD and MAH received a scholarship from CONACYT.