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Aseptic Conditions to Reduce Risks to Metabolic Fidelity
Author(s) -
Henn Alicia,
He Yan,
Darou Shan,
Yerden Randy
Publication year - 2020
Publication title -
the faseb journal
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.709
H-Index - 277
eISSN - 1530-6860
pISSN - 0892-6638
DOI - 10.1096/fasebj.2020.34.s1.04013
Subject(s) - aseptic processing , antibiotics , microbiology and biotechnology , contamination , bacteria , in vitro , metabolic activity , biology , cell , food science , physiology , biochemistry , ecology , genetics
Mitochondria are derived from bacteria, which puts them at risk of stress from antibiotics commonly used in cell culture. Antibiotics have been found to cause metabolic changes in cells in vitro, fundamentally altering cellular function and response. We have been culturing without antibiotics for over 8 years using the aseptic conditions in the closed Xvivo System and we have not had a single culture contaminated. With the hypothesis that routine disinfection and the conditions inside the chambers would manage microbial risks without antibiotics, we performed routine cell culture processes (cell thaw, cell passage, cell harvest) using highly permissive color‐changing TSB bacterial broth. In 4 trials, no contamination was found of any mock cultures. Monitoring of the environment inside the system with bacterial contact plates showed no evidence of contamination. Positive and negative controls showed appropriate growth. Our results showed that adding antibiotics is not necessary for aseptic conditions of cell culture, which highly reduces the metabolic risk to cells and would enhance normal cellular functions and responses in vitro.