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Effect of Mesenchymal Stromal Cells (MSC) and Platelet‐Rich Plasma (PRP) on Rat Achilles Tendon Repair
Author(s) -
Fagan Diana,
Austin Brittany,
Drew Stuart,
Etty Thywill,
Juzbasich Dragan,
Pham Errek,
Marie Hazel
Publication year - 2020
Publication title -
the faseb journal
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.709
H-Index - 277
eISSN - 1530-6860
pISSN - 0892-6638
DOI - 10.1096/fasebj.2020.34.s1.03927
Subject(s) - achilles tendon , platelet rich plasma , medicine , tendon , mesenchymal stem cell , fibrous joint , achilles tendon rupture , surgery , calcaneus , platelet , pathology
Achilles tendon rupture requires long recovery times and has the possibility of re‐rupture. The goal of this project is to improve healing in surgically repaired Achilles tendons in a Lewis rat model. Full thickness surgical transections were made 6 mm proximal to the calcaneus bone. Following suture repair, a 20 x 30 mm piece of Collatape TM was wrapped around the tendon in groups receiving collagen and the skin closed. MSC or PRP or both (100 ml) was injected subcutaneously adjacent to the incision. Both test Achilles and control Achilles from the same animal were harvested and the biomechanical properties determined using a standard force‐extension analysis of tensile properties after 1 week or 2 weeks. A significant improvement in Strain at UTS and Strain at Failure (calculated as percent of control tendon) was seen at 1 week in rats receiving Collatape TM , plus PRP and MSC. This project was approved by the Northeast Ohio Medical School IACUC. Support or Funding Information Supported by Mercy Health, Youngstown and Youngstown State University.