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Development of substitute of R‐spondin 1 for production of prostate organoid
Author(s) -
Cha Ji Hyeon,
Heo Hee Sun,
Lee Seung Hwan,
Lee Jong Hun
Publication year - 2020
Publication title -
the faseb journal
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.709
H-Index - 277
eISSN - 1530-6860
pISSN - 0892-6638
DOI - 10.1096/fasebj.2020.34.s1.03874
Subject(s) - organoid , lgr5 , wnt signaling pathway , prostate , biology , microbiology and biotechnology , medicine , signal transduction , cancer
Organoid that is organ‐like cell aggregates has both the advantages of 'in vitro' and three‐dimensional advantages of animal experiment. It is very useful for modeling disease, drug development research, and testing customized medicine. R‐spondin 1 stimulates Wnt / β‐catenin signaling to induce proliferation of organoids. Thus, high concentrations of R‐spondin 1 are essential for organoid culture. However, production of R‐spondin 1 is limited and, due to the high cost of R‐spondin 1, there are financial difficulties in the practical application of organoids. We have found an alternative to the R‐spondin 1 protein from natural compounds. We also evaluated how this natural component affects prostate organoid. We confirmed that prostate organoids were established from selected natural products. Cultures were divided into Group A with R‐spondin 1, Group B with natural products and Group C with R‐spondin 1 and natural products. In order to confirm whether organoids actually have prostate tissue characteristics, we confirmed expression of basal specific markers and, lumen specific markers including prostate specific antigen (PSA). Cyrokeratin 8 and PSA were used as luminal specific markers, and p63 and cytokeratin 5 were used as basal specific markers. This study showed that R‐spondin 1, Lgr5 antagonist, could be replaced by natural compound in organoid culture. We also could observe that natural‐derived components helped R‐spondin 1 to promote growth of prostate organoid and inhibit apoptosis. This research is expected to contribute to the research of organoids that will be developed in the future.