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Analysis of Platelet Aggregation, Secretion, Integrin Activation, and Calcium Release Stimulated by Different Agonists in Native Americans
Author(s) -
Williquett Brady,
Valen Grace E.,
Goerger Krista,
Clarke Santa,
Abbott Karla,
Larson Mark K.
Publication year - 2020
Publication title -
the faseb journal
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.709
H-Index - 277
eISSN - 1530-6860
pISSN - 0892-6638
DOI - 10.1096/fasebj.2020.34.s1.03416
Subject(s) - platelet , platelet activation , medicine , thrombosis , population , integrin , immunology , endocrinology , pharmacology , receptor , environmental health
Arterial thrombosis results from obstructive vascular blood clots that are largely initiated by platelet activation. As such, individuals whose platelets react more robustly to chemical signals generated by vascular damage or dysfunction are at a higher risk for morbidity and mortality caused by thrombosis. As a population, Native Americans have higher rates of obstructive arterial diseases, and previous research suggests African American populations have higher overall platelet reactivity that positively correlates to their higher risk for cardiovascular disease. Therefore, we set out to measure platelet reactivity in Native Americans and identify potential genetic alleles that correlate to the elevations in responsivity. Five platelet agonists were utilized to simulate vascular damage signals, followed by measurements of markers indicative of subsequent platelet activation: aggregation, secretion, integrin activation, and calcium ion mobilization. Preliminary results from 17 subjects showed that Native American platelets more rapidly aggregate in response to several agonists when compared with Caucasians, whereas other measures of platelet function are largely similar between the groups. These results suggest that Native Americans have more sensitive platelet aggregation responses, which could result in a higher risk for occlusive arterial diseases. Complete genomic sequences were also obtained from the highest responders for use in identifying alleles that correlate to this response. Support or Funding Information Institutional Development Award (IDeA) from the National Institute of General Medical Sciences of the National Institutes of Health under grant number P20GM103443