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MicroRNA‐200a‐3p mediates neuroprotection in Alzheimer‐related deficits and attenuates amyloid‐beta overproduction and tau hyperphosphorylation via co‐regulating BACE1 and PRKACB
Author(s) -
Liu Rui,
Wang Lin-lin,
Jiang Hai-lun
Publication year - 2020
Publication title -
the faseb journal
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.709
H-Index - 277
eISSN - 1530-6860
pISSN - 0892-6638
DOI - 10.1096/fasebj.2020.34.s1.03216
Subject(s) - neuroprotection , hyperphosphorylation , microbiology and biotechnology , tau protein , amyloid precursor protein , kinase , amyloid precursor protein secretase , chemistry , downregulation and upregulation , protein kinase a , amyloid beta , western blot , phosphatase , biology , phosphorylation , alzheimer's disease , biochemistry , pharmacology , medicine , peptide , disease , gene
Alzheimer’s disease (AD) is characterized by two landmark pathologies, the overproduction of amyloid‐beta peptides (Aβ), predominated by the β‐amyloid protein precursor cleaving enzyme 1 (BACE1), and hyperphosphorylation of the microtubule protein, tau, due to an imbalance in a kinase / phosphatase system that involves the activation of the protein kinase A (PKA). Current evidence indicates that brain microRNAs participate in multiple aspects of AD pathology. Here, the role and underlying molecular mechanisms of microRNA‐200a‐3p (miR‐200a‐3p) in mediating neuroprotection against AD‐related deficits were investigated. The expression of miR‐200a‐3p was measured in the hippocampus of APP / PS1 and SAMP8 mice and in an AD cell model in vitro , as well as in blood plasma extracted from AD patients. The targets of miR‐200a‐3p were determined using bioinformatics and dual‐luciferase assay analyses. In addition, cell apoptosis was detected using flow cytometry and related protein levels were measured using Western blot and ELISA techniques. miR‐200a‐3p was confirmed to be depressed in microarray miRNA profile analysis in vitro and in vivo , suggesting that miR‐200a‐3p is a potential biomarker of AD. Subsequently, miR‐200a‐3p was demonstrated to inhibit cell apoptosis accompanied by the inactivation of the Bax / caspase‐3 axis and downregulation of Aβ 1‐42 and tau phosphorylation levels in vitro . Further mechanistic studies revealed that miR‐200a‐3p reduced the production of Aβ 1‐42 and decreased hyperphosphorylation of tau by regulating the protein translocation of BACE1 and the protein kinase cAMP‐activated catalytic subunit beta ( PRKACB ) associated with the three prime untranslated regions (3’‐UTRs), respectively. Importantly, the function of miR‐200a‐3p was reversed by overexpression of BACE1 or PRKACB in cultured cells. This resulted in an elevation in cell apoptosis, and an increase in Aβ 1‐42 , and tau hyperphosphorylation levels, involving the epitopes, threonine 205, serine 202, 214, 396, and 356, the favorable phosphorylated sites of PKA. In conclusion, our study suggests that miR‐200a‐3p is implicated in the pathology of AD, exerting neuroprotective effects against Aβ‐induced toxicity by two possible mechanisms: one involving the inhibition of Aβ overproduction via suppressing the expression of BACE1 and; two, synergistically, decreasing the hyperphosphorylation of tau via attenuating the expression of PKA. Support or Funding Information This study was supported by the National NaturalScience Foundation of China (No. U1803281 and 81673411), China; the Non‐profitCentral Research Institute Fund of Chinese Academy of Medical Sciences(2018RC350013), China; and Chinese Academy Medical Sciences (CAMS) InnovationFund for Medical Science (2017‐I2M‐1‐016), China.