Premium
CYTOTOXIC POTENTIALS OF METHANOL EXTRACTS OF EKLONIA RADIATA AND JANIA VERUCOSA IN DIFFERENT CANCER CELLS AND NON‐CANCEROUS CELL LINE.
Author(s) -
SAIBU GBEMISOLA MOROUNKE,
Nakin Motebang Dominic,
Oyedeji Adebola Omowunmi,
Oyedeji Opeoluwa,
Meyer Mervin
Publication year - 2020
Publication title -
the faseb journal
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.709
H-Index - 277
eISSN - 1530-6860
pISSN - 0892-6638
DOI - 10.1096/fasebj.2020.34.s1.02837
Subject(s) - cytotoxicity , chemistry , mtt assay , cell culture , viability assay , formazan , cell , traditional medicine , biochemistry , biology , medicine , in vitro , genetics
Finding novel anticancer agents is very important for cancer treatment. Marine organisms are valuable source for developing novel agents for clinical applications. Seaweeds have been shown to have several biological activities, including anticancer activity. This study aimed at evaluating the cytotoxicity potentials of methanol extracts of Eklonia radiata (ER) and Jania verucosa (JV). Materials and Methods In this study, a panel of three cancer cell lines (HepG2, MDA and CaCo2) and one non‐cancerous cell line (KMST‐6) were treated with methanol extracts of Eklonia radiate (ER) and Jania verucosa (JV) . Both ER and JV were found on rocky shores of the Eastern coast of South Africa, ER belongs to Kelp family while JV belongs to Corallinaceae family. The cells were treated with various concentrations of methanol extracts of ER and JV for 24 hrs, the percentage viability values of the cells were determined by the tetrazolium salt reduction assay (MTT) .Results Morphological alteration of the cell lines after exposure with methanol extracts of Ecklonia radiata (ER) and Janica verucosa (JV) were observed under phase contrast microscope. The cytotoxicity result showed that cells treated with both methanol extracts of ER and JV had more cytotoxicity effect on MDA treated cells followed by HepG2 and Caco2. Non‐cancerous cell line (KMST‐6) however showed some selectivity in their activities. Conclusions Seaweeds generally change estrogen metabolism. However, in this present study, it can be established that both Eklonia radiate (ER) and Jania verucosa (JV) had potential for chemoprevention of human breast and this can be considered as potential sources of anticancer agent. Further studies are necessary for isolation and identification of biologically active substances present in these extracts.