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Antioxidant Activities of Green Synthesized Zinc Oxide Nanoparticles and its Curative Influence in Carbon tetrachloride‐Induced Hepatorenal Damage in Adult Rats
Author(s) -
Otuechere Chiagoziem A.,
Ekozin Adriel,
Adewuyi Adewale
Publication year - 2020
Publication title -
the faseb journal
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.709
H-Index - 277
eISSN - 1530-6860
pISSN - 0892-6638
DOI - 10.1096/fasebj.2020.34.s1.02637
Subject(s) - chemistry , carbon tetrachloride , antioxidant , glutathione , ascorbic acid , glutathione peroxidase , superoxide dismutase , biochemistry , alkaline phosphatase , abts , uric acid , pharmacology , medicine , nuclear chemistry , dpph , food science , organic chemistry , enzyme
The objective of this study was to synthesize Zinc Oxide nanoparticles coated with Pterocarpus mildbraedii leaf extracts (PmZnONPs) by an eco‐friendly method, and investigate its antioxidant activity and curative potential against carbon tetrachloride (CCl 4 ) ‐ induced hepatorenal toxicity in rats. PmZnONPs was characterized by transmission electron microscopy (TEM), scanning electron microscopy (SEM), fourier transform infra‐red spectroscopy (FTIR), alongside zeta potential measurement. In vitro , free radical activities using 1, 1‐diphenyl‐2‐ picrylhydrazyl (DPPH) and 2,2′‐azino‐bis(3‐ethylbenzothiazoline‐6‐sulfonic acid (ABTS) showed strong antioxidant activity compared to ascorbic acid. In rats, PmZnONPs (1 and 3 mg/kg p.o.) significantly prevented CCl 4 ‐induced elevations of alkaline phosphatase, gamma‐glutamyl transferase, creatinine, urea, aspartate aminotransferase, alanine aminotransferase, and total bilirubin. Moreover, PmZnONPs restored the decreased levels of plasma uric acid, hepatorenal antioxidant enzymes, including superoxide dismutase, glutathione peroxidase, glutathione transferase, and glutathione, that were significantly induced by CCl 4 treatment. Histological studies indicated that CCl 4 ‐induced lesions in the liver and kidney were alleviated by the treatment of PmZnONPs. Immunohistochemical studies showed that PmZnONPs significantly suppressed the high immunoreactivity of cyclooxygenase‐2, interleukin‐6 and NF‐κB arising from CCl 4 intoxication. These results indicated that PmZnONPs protected the rats from CCl 4 ‐induced hepatorenal damage via its antioxidant properties as well as its inhibition of pro‐inflammatory proteins. Support or Funding Information This research did not receive any support from any funding organization.