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Antioxidant activity of the ethyl acetate extract of Justicia spicigera in two periods of the year (spring‐summer and autumn‐winter) in the liver of adult diabetic rats
Author(s) -
Murillo-Villicaña Marina,
Peña-Montes Donovan J.,
Huerta-Cervantes Maribel,
Salgado-Garciglia Rafael,
Noriega-Cisneros Ruth,
Aguilera-Méndez Asdrúbal,
Saavedra-Molina Alfredo
Publication year - 2020
Publication title -
the faseb journal
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.709
H-Index - 277
eISSN - 1530-6860
pISSN - 0892-6638
DOI - 10.1096/fasebj.2020.34.s1.02526
Subject(s) - catalase , diabetes mellitus , streptozotocin , glutathione , superoxide dismutase , antioxidant , medicine , endocrinology , ethyl acetate , insulin , zoology , oxidative stress , chemistry , biochemistry , biology , enzyme
Background Diabetes is a chronic disease that occurs either when the pancreas does not produce enough insulin or when the body cannot effectively use the insulin it produces. In studies it was shown that the ethanol extract of Justicia spicigera has a hypoglycemic effect in streptozotocin‐induced rats (STZ), related to the presence of secondary metabolites, such as kaempferitrin. However, the concentration of these compounds presents drastic variations under different environmental conditions. Objective The objective of this project is to study the activity of secondary metabolites produced by Justicia spicigera during two periods of the year (spring‐summer and autumn‐winter) in a chronic diabetes model on the liver of streptozotocin‐induced rats. Methods Male Wistar rats (450–500 g) with 20 weeks of age were used, divided into 6 groups: normoglycemic control (CN) and diabetic (CD), administered with spring‐summer extract (NEPV and DEPV) and autumn‐winter ( NEOI and DEOI) for 60 days, diabetic rats were induced with an intraperitoneal injection of STZ (45 mg / kg). During treatment the weight and blood glucose were determined every 6 days. Subsequently, the rats were submitting euthanasia to obtain weight, homogenate and mitochondria of liver, which were used for the determination of catalase and superoxide dismutase activities and glutathione levels. Results The ethyl acetate extract of Justicia Spicigera collected during the spring‐summer season significantly lowers blood glucose levels and prevents excessive loss of body weight and liver growth increases catalase activity and reduced glutathione levels. The ethyl acetate extract of Justicia Spicigera collected during the autumn‐winter season prevents excessive loss of body weight and liver growth, but does not exhibit antioxidant activity when compared to the DEPV group. In conclusion, the concentration of Justice spicigera compounds related to hypoglycemic and antioxidant activity is affected by environmental factors. Support or Funding Information The authors appreciate the partial economic support from CIC‐UMSNH (2.16, to ASM).