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Improved Difluoroboron β‐Diketonate Poly(lactic acid) Nanoparticles for Monitoring Wound Oxygenation
Author(s) -
Bruce Anthony C.,
Zhuang Meng,
DeRosa Christopher A.,
Corliss Bruce A.,
Fraser Cassandra L.,
Peirce Shayn M.
Publication year - 2020
Publication title -
the faseb journal
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.709
H-Index - 277
eISSN - 1530-6860
pISSN - 0892-6638
DOI - 10.1096/fasebj.2020.34.s1.02463
Subject(s) - fluorescence , oxygen , rgb color model , nanoparticle , lactic acid , phosphorescence , wound healing , chemistry , biomedical engineering , materials science , fluorescence microscope , nanotechnology , optics , surgery , computer science , medicine , organic chemistry , bacteria , physics , biology , genetics , operating system
We have previously demonstrated the ability of a difluoroboron β‐diketonate poly(lactic acid) material, BF 2 nbm(I)PLA, hereafter nbm, to detect tissue oxygen levels in murine cutaneous wound beds 1. This relies on oxygen‐insensitive fluorescence (F) as an internal standard and oxygen‐sensitive phosphorescence (P) as a sensor. In an effort to improve imaging agent clearance and ease of manufacture, as well as to optimize emission wavelengths to color channels of RGB cameras, a new dithienyl dye, BF 2 dtm(I)C 12 H 25‐ , hereafter dtm, with red phosphorescence was developed. The dtm dye was mixed with a blue fluorescent dye, BF 2 dbm(F)OCH 12 H 25 , hereafter dbm, and coprecipitated with PLA to form oxygen sensing nanoparticles. The objective of this study was to verify the utility of the newly developed dtm/dbm nanoparticles for sensing tissue oxygen levels in murine wound beds. To achieve this, we monitored the relative tissue oxygen levels of healing full‐thickness cutaneous wounds in C57BL/6J mice over the course of one week with both dtm/dbm and nbm using an RGB camera mounted to a microscope and custom MATLAB software. We found the new dtm/dbm nanoparticles yielded nearly identical trends over time in wound bed F/P versus nbm. The dtm/dbm nanoparticles did not impair wound closure and demonstrated less adhesion to healing tissues relative to nbm. Dtm/dbm retains the tissue oxygen‐sensing capability of nbm while having fewer synthetic steps, better phosphorescence alignment with red RGB channel, improved solubility, and less retention in wound tissue than nbm. Support or Funding Information UVA‐Coulter Translational Research Project #155234Verification of dtm/dbm in murine cutaneous wounds A. Dtm/dbm generated the same trends in wound bed tissue oxygenation over time as nbm. B. Dtm/dbm did not impair wound healing versus nbm or saline control. C. Dtm/dbm displayed reduced particle retention in wounds after multiple applications. Scale bar = 1 mm1 DeRosa , C. A. ; Seaman , S. A. ; Mathew , A. S. ; Gorick , C. M. ; Fan , Z. ; Demas , J. N. ; Peirce , S. M. ; Fraser , C. L. Oxygen Sensing Difluoroboron β-Diketonate Polylactide Materials with Tunable Dynamic Ranges for Wound Imaging . ACS Sensors 2016 , 1 , 1366 – 1373 .