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Genome‐wide Association Study of Sperm Resistance to Freezing in Horses
Author(s) -
Nikitkina Elena,
Kudinov Andrey,
Atroshchenko Mikhail,
Dementeva Natalia,
Pozovnikova Marina,
Larkina Tatiana,
Shcherbakov Yuri,
Krutikova Anna,
Musidray Artem
Publication year - 2020
Publication title -
the faseb journal
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.709
H-Index - 277
eISSN - 1530-6860
pISSN - 0892-6638
DOI - 10.1096/fasebj.2020.34.s1.02298
Subject(s) - semen , sperm , genome wide association study , biology , genotyping , cryopreservation , single nucleotide polymorphism , snp , andrology , genetics , genome , genetic association , semen analysis , gene , genotype , embryo , infertility , medicine , pregnancy
The use of cryopreserved sperm has become a routine in horse breeding. Genetic factors affecting cryoresistance remain poorly understood. The aim of the study was to search for genome‐wide associations with a decreasing in sperm motility after freezing. Semen of 96 horses was frozen. Sperm motility was evaluated by computer‐assisted semen analysis (CASA)( ArgusSoft, Russia) before and after freezing. The evaluation was carried out using an Axio Imager microscope (Carl Zeiss Microscopy GmbH, Germany). DNA was isolated from sperm samples for genotyping on Affymetrix Equine HD array 96 SNP chips. Calculations were performed using the PLINK and EMMAX programs. The Genome‐Wide Association Studies (GWAS) was used for the analysis of genomic associations. As a result 45 significant associations were found. 14 are on the first chromosome in region 1: 166861063‐167517944 in the vicinity of the LOC11769038 gene. It is assumed that the polymorphisms found are associated with the formation of functional resistance of spermatozoa to cryopreservation. Studies have opened up the possibility of studying the changes in the cells after freezing. Found SNPs markers will be used to select males whose semen is suitable for cryopreservation. Support or Funding Information The study was supported by the Russian Science Foundation (project No. 18‐16‐00071)Manhattan plot of GWAS