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The Effects of C‐Phycocyanin and Lycium barbarum Polysaccharides on Aspirin‐Induced Apoptosis in Rat Gastric Mucosal Cells
Author(s) -
Chao Jane C-J,
Liu Yu-Chen,
Hsu Chien-Yeh,
Chen Yu-Shan
Publication year - 2020
Publication title -
the faseb journal
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.709
H-Index - 277
eISSN - 1530-6860
pISSN - 0892-6638
DOI - 10.1096/fasebj.2020.34.s1.02288
Subject(s) - apoptosis , aspirin , chemistry , pharmacology , antioxidant , p38 mitogen activated protein kinases , lycium , medicine , kinase , biochemistry , protein kinase a , pathology , alternative medicine
Non‐steroidal anti‐inflammatory drugs (NSAIDs) cause gastrointestinal injury through both topical and systemic effects, which may generate reactive oxygen species and lead to apoptosis. Aspirin, a NSAID, is widely used for cardio‐protection and causes gastrotoxicity in long‐term administration. C‐phycocyanin (CPC) is a biliprotein pigment of the blue‐green alga Spirulina platensis , and possesses antioxidant, anti‐inflammatory, and wound‐healing properties. Lycium barbarum polysaccharides (LBP) extracted from wolfberry act as an antioxidant and immunomodulator. This study investigated the effects of CPC and LBP on aspirin‐induced apoptosis in rat gastric mucosal RGM‐1 cells. Rat RGM‐1 cells were pretreated with different concentrations of CPC and/or LBP (100, 250, and 500 μg/mL) for 24 hours, and gastric damage was induced by 21 mM aspirin for 3 hours. Apoptotic protein expression was determined by sodium dodecyl sulfate polyacrylamide gel electrophoresis and Western blotting. The results showed that aspirin significantly increased c‐Jun N‐terminal kinase (JNK), p38 phosphorylation, and Bax expression, while LBP decreased JNK activation and Bax expression compared with aspirin treatment alone. However, CPC had no effects on these apoptotic markers. In conclusion, LBP may decrease aspirin‐induced apoptosis in rat gastric mucosal RGM‐1 cells by suppressing the activation of JNK signaling pathway. Support or Funding Information The study was supported by the Ministry of Science and Technology, Taiwan (grant no. MOST105‐2320‐B‐038‐036‐MY3).