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Examination of Cortactin‐ and Membrane‐Associated Proteins at Listeria Membrane Protrusions
Author(s) -
Bruzzini Kaitlin M.,
Guttman Julian A.
Publication year - 2020
Publication title -
the faseb journal
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.709
H-Index - 277
eISSN - 1530-6860
pISSN - 0892-6638
DOI - 10.1096/fasebj.2020.34.s1.02231
Subject(s) - cortactin , microbiology and biotechnology , listeria monocytogenes , membrane ruffling , intracellular , actin , biology , actin cytoskeleton , cytoskeleton , cell , chemistry , bacteria , biochemistry , genetics
Listeria monocytogenes is an intracellular pathogen that manipulates its host’s cytoskeleton to polymerize actin together with an array of actin‐associated proteins, including VASP and cortactin to one pole of the bacteria forming comet/rocket tails for intracellular propulsion. These structures also enable the microbes to move from one cell to another through a cell‐to‐cell spreading process involving the bacteria pressing into the host’s plasma membrane, forming L. monocytogenes protrusions which are internalized into corresponding invaginations in neighbouring cells. Recently, a siRNA screen listed genes involved in comet/rocket tail formation and cell‐to‐cell spreading. Here we examined a subset of those proteins (Endophilin, Ephrin A2, Anti‐Ephrin B1) as well as cortactin‐associated proteins (FGD1, SHK, VAV2) by immunolocalization at actin‐rich structures generated during L. monocytogenes infections.