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Yiqi‐Huoxue Granule suppresses inflammation induced by Lipopolysaccharide in mice by inhibiting the activation of NF‐κB pathway
Author(s) -
WU HONG,
Wang Xinzhou,
Gao Shuibo,
Dai Liping,
Wang Zhentao,
Han Lihua
Publication year - 2020
Publication title -
the faseb journal
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.709
H-Index - 277
eISSN - 1530-6860
pISSN - 0892-6638
DOI - 10.1096/fasebj.2020.34.s1.02189
Subject(s) - lipopolysaccharide , nf κb , phosphorylation , inflammation , western blot , tumor necrosis factor alpha , chemistry , vcam 1 , medicine , iκbα , endocrinology , icam 1 , immunology , biochemistry , cell adhesion molecule , gene
Objective To study the effects of Yiqi Huoxue Granule (YQHX) on inflammatory responses and endothelial injury induced by lipopolysaccharide (LPS) in mice. Methods Male C57BL/6 mice (8–12 weeks old) were divided into five groups, including control group, LPS group, YQHX group, PDTC (NF‐κB inhibitor) group, and PDTC plus YQHX group. After orally administered with YQHX for 2 weeks or intraperitoneally injected with PDTC for 1 h, LPS was injected into mice for another 6 h, and serums and aortas were collected, respectively. The levels of TNF‐α and IL‐6 in the serum were detected using ELISA. The mRNA expression levels of VCAM‐1, ICAM‐1, PAI‐1 and TF were determined using qRT‐PCR and the protein levels of these factors as well as the phosphorylation levels of NF‐κB pathway were detected using Western blot. Results The levels of TNF‐α, IL‐6, VCAM‐1, ICAM‐1, PAI‐1 and TF in LPS group were higher than those in control group ( P <0.01), and the phosphorylation levels of P65 and IκB were also increased significantly ( P <0.01). Compared with the LPS group, the levels of TNF‐α, IL‐6, VCAM‐1, ICAM‐1, PAI‐1, TF and NF‐κB pathway phosphorylation were decreased in YQHX group, PDTC group and YQHX plus PDTC group ( P <0.05, P <0.01). There was no significant difference among groups of PDTC and YQHX plus PDTC ( P >0.05). Conclusion YQHX suppresses the inflammatory responses and endothelial injuries induced by LPS in mice by inhibiting the activation of NF‐κB pathway. Support or Funding Information This work was financially supported by grants from the National Natural Science Foundation of China (Grant No. 81473453 & 81673800)