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Engineering Temperature Sensitive Alleles in Saccharomyces cerevisiae to Study the Genetic Interactions Between Ran point mutants and its Accessory Proteins
Author(s) -
Tsepal Tenzin
Publication year - 2020
Publication title -
the faseb journal
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.709
H-Index - 277
eISSN - 1530-6860
pISSN - 0892-6638
DOI - 10.1096/fasebj.2020.34.s1.02170
Subject(s) - ran , mutant , saccharomyces cerevisiae , gtpase , small gtpase , biology , gtpase activating protein , gene , genetics , point mutation , escrt , microbiology and biotechnology , g protein , signal transduction , cell , endosome
Ran is an essential GTPase protein involved in bidirectional transportation between nucleus and cytoplasm. We engineered temperature sensitive (TS) mutant alleles of Ran accessory proteins in S. cerevisiae to study the genetic interactions. For essential proteins like Ran and its accessory proteins, TS mutants give insight into gene function without knocking out genes. By creating these TS mutant strains, we will be able to study the genetic interactions between Ran point mutants and TS mutant Ran accessory proteins to closely interrogate the biophysics and mechanism of the Ran GTPase cycle and it’s role nucleocytoplamsic transport. Support or Funding Information The 2019 NIH Summer Undergraduate Research Program at UMass Medical School. NIH Grant # R25 HL092610‐11