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Methylphenidate Does Not Hypersensitize the Rat Heart to Ischemic Injury
Author(s) -
Rorabaugh Boyd R.,
Seeley Sarah L.,
Stoop Thorne S.,
D'Souza Manoranjan S.
Publication year - 2020
Publication title -
the faseb journal
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.709
H-Index - 277
eISSN - 1530-6860
pISSN - 0892-6638
DOI - 10.1096/fasebj.2020.34.s1.02155
Subject(s) - methylphenidate , medicine , anesthesia , ischemia , cardiology , methamphetamine , dopamine , myocardial infarction , dopaminergic , attention deficit hyperactivity disorder , psychiatry
Background Methylphenidate is commonly used for the treatment of attention deficit hyperactivity disorder. The cardiovascular safety of methylphenidate has been a subject of debate with some studies indicating that methylphenidate increases the likelihood of experiencing a myocardial infarction. However, it is unknown whether methylphenidate alters the extent of myocardial injury during an ischemic insult. Previous work demonstrated that a history of methamphetamine exposure increases infarct size and postischemic recovery of contractile function in the ischemic heart in a sex‐dependent manner. Methylphenidate and methamphetamine both increase synaptic concentrations of dopamine and norepinephrine, resulting in enhanced adrenergic and dopaminergic signaling. However, it is unknown whether methylphenidate also hypersensitizes the heart to ischemia. The purpose of this study was to determine whether methylphenidate increases the extent of myocardial injury during an ischemic insult. Methods Male and female rats were treated with methylphenidate (5 mg/kg/day) or saline for 10 days. Hearts were subjected to a 20 min ischemic insult and 2 hours of reperfusion on a Langendorff isolated heart apparatus on day 11. Cardiac contractile function was monitored via an intraventricular balloon, and myocardial injury was assessed by triphenyltetrazolium chloride staining. Results Methylphenidate significantly increased locomotor activity in male and female rats. Male hearts had significantly larger infarcts than female hearts, but methylphenidate had no impact on infarct size or postischemic recovery of contractile function in hearts of either sex. Conclusion These data indicate that methylphenidate does not increase myocardial sensitivity to ischemic injury. Support or Funding Information This work was supported by 1R15HL145546 to BRR.