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SPARC, a Muscle‐Secreted Protein, Improves Glucose Tolerance via AMP‐activated Protein Kinase in Skeletal Muscle
Author(s) -
Aoi Wataru,
Sakuma Kunihiro,
Krook Anna
Publication year - 2020
Publication title -
the faseb journal
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.709
H-Index - 277
eISSN - 1530-6860
pISSN - 0892-6638
DOI - 10.1096/fasebj.2020.34.s1.02102
Subject(s) - myokine , ampk , skeletal muscle , glucose uptake , protein kinase a , endocrinology , medicine , carbohydrate metabolism , amp activated protein kinase , metabolism , chemistry , kinase , biology , microbiology and biotechnology , biochemistry , insulin
Secreted protein acidic and rich in cysteine (SPARC) is an exercise‐responsive myokine. Although SPARC has been implicated in nutrient metabolism, the effect of this myokine on muscle metabolism is unclear. Here we investigated the effect of SPARC on glucose metabolism in skeletal muscle. SPARC‐knockout mice showed impaired systemic glucose metabolism. Chronic SPARC treatment improved systemic glucose tolerance and AMP‐activated protein kinase (AMPK) signaling in skeletal muscle of high‐fat diet‐induced obese mice. A protein interaction assay showed that SPARC strongly interacted with voltage dependent calcium channel. In cell culture experiments, SPARC increased glucose metabolism concomitant with activation of AMPK, and this effect was suppressed by inhibiting the calcium‐dependent signaling. Our findings suggest that SPARC treatment mimics the effects of exercise on glucose tolerance by enhancing AMPK‐dependent glucose uptake in skeletal muscle. Support or Funding Information This study was partly supported by the Japan Society for the Promotion of Science (JSPS) KAKENHI: Grant‐in‐Aid for Scientific Research (B) (grant numbers 25282199 and 17H02176).