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Regulation of Histone Gene Expression during DNA Damage Response in Budding Yeast
Author(s) -
Bhagwat Madhura,
Nagar Shreya,
Kaur Pritpal,
Vancura Ales
Publication year - 2020
Publication title -
the faseb journal
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.709
H-Index - 277
eISSN - 1530-6860
pISSN - 0892-6638
DOI - 10.1096/fasebj.2020.34.s1.01953
Subject(s) - histone , dna damage , histone h2a , microbiology and biotechnology , dna repair , biology , chromatin , histone methylation , gene expression , gene , chemistry , dna , genetics , dna methylation
The DNA damage response (DDR), a highly conserved process, senses and repairs damaged DNA. We have showed previously that DDR activates respiration to increase ATP production and to elevate dNTP levels, which are required for efficient DNA repair and cell survival upon DNA damage. The underlying mechanism involves DNA damage‐induced downregulation of histone expression and altered chromatin structure, leading to increased transcription of genes encoding enzymes of tricarboxylic acid cycle, electron transport chain, and oxidative phosphorylation. Here we show that the downregulation of histone gene expression during DDR requires Rad53p and occurs at the level of transcription and not at the level histone mRNA decay. The corresponding mechanism involves DDR‐mediated inhibition of assembly of the preinitiation complex and decreased recruitment of Spt10p and Spt21p to the promoters of histone genes. Support or Funding Information NIH GM120710