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GlycoSense&[trade]: A Flow Cytometry‐Based Technology for Rapid and Simplified Glycan Profiling
Author(s) -
Saunders Matthew,
Woods Robert J.,
Yang Loretta
Publication year - 2020
Publication title -
the faseb journal
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.709
H-Index - 277
eISSN - 1530-6860
pISSN - 0892-6638
DOI - 10.1096/fasebj.2020.34.s1.00483
Subject(s) - glycan , multiplex , glycosylation , glycoprotein , chemistry , reagent , flow cytometry , computational biology , biochemistry , microbiology and biotechnology , biology , bioinformatics , organic chemistry
Lectenz Bio has developed an innovative technology called GlycoSense™ that detects binding between glycans and glycan‐specific reagents that are conjugated to spectrally‐unique beads. By combining the individual bead‐based reagents into a multiplex array, a profile of key glycan features can be obtained in a few minutes on a basic cytometer after an incubation step. We illustrate the performance of the method using glycoprotein standards and demonstrate its utility by monitoring changes in terminal glycosylation and linkages upon treatment with glycoenzymes. The GlycoSense™ approach addresses the unmet need for a rapid and robust tool to analyze protein glycosylation. In addition to established carbohydrate‐detection reagents such as antibodies and lectins, this approach can employ the engineered proteins (known as Lectenz ® ) developed by Lectenz Bio. Support or Funding Information NIH U01CA221213, R43GM123863