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Illuminating the Biochemical Activity Architecture of the Cell
Author(s) -
Zhang Jin
Publication year - 2020
Publication title -
the faseb journal
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.709
H-Index - 277
eISSN - 1530-6860
pISSN - 0892-6638
DOI - 10.1096/fasebj.2020.34.s1.00385
Subject(s) - signal transduction , mtorc1 , pi3k/akt/mtor pathway , second messenger system , biosensor , kinase , microbiology and biotechnology , protein kinase b , cell signaling , phosphatase , computational biology , biology , chemistry , biochemistry , phosphorylation
The complexity and specificity of many forms of signal transduction are widely suspected to require spatial microcompartmentation and dynamic modulation of the activities of signaling molecules, such as protein kinases, phosphatases and second messengers. We have developed a series of fluorescent biosensors to probe the compartmentalized signaling activities in living cells. In this talk, I will focus on cAMP/PKA and PI3K/Akt/mTORC1 signaling pathways and present studies where we combined genetically encoded fluorescent biosensors, superresolution imaging, targeted biochemical perturbations and mathematical modeling to probe the biochemical activity architecture of the cell. Support or Funding Information The work is supported by NIH