Premium
Human Skeletal Muscle‐on‐a‐Chip
Author(s) -
Naik Akshata R,
Pernal Sebastian,
Lewis Kenneth T,
Wu Yaobin,
Wu Hongkai,
Carruthers Nicholas J,
Stemmer Paul,
Jena Bhanu P
Publication year - 2019
Publication title -
the faseb journal
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.709
H-Index - 277
eISSN - 1530-6860
pISSN - 0892-6638
DOI - 10.1096/fasebj.2019.33.1_supplement.lb645
Subject(s) - skeletal muscle , myocyte , microbiology and biotechnology , in vitro , mitochondrial biogenesis , biology , chemistry , anatomy , mitochondrion , biochemistry
A comprehensive understanding of the emergent properties of in vitro skeletal muscle growth and development is still in its infancy. To address this gap in our current knowledge, we have developed a stretchable micropatterned 3D human skeletal muscle platform that recapitulates organized and parallel growth of muscle cells and fibers as opposed to the randomly oriented cells growth on a 2D glass surface. Mass spectrometry of the muscle cells growing on the 3D platform express key myogenic proteins for myoblast fusion required in the formation of muscle tissue, and proteins involved in mitochondrial health and biogenesis, in contrast to cells growing on 2D glass surface. These results demonstrate that the engineered human muscle cells grown on the 3D platform holds great promise to further establish the emergent properties of in vitro skeletal muscle growth and development for a wide range of biomedical applications such as transplants, drug screening and gene therapy. Support or Funding InformationInterdisciplinary Biological Sceinces (IBS) fellowship Graduate Research Assistantship, Department of Physiology Richard Barber Fellowship National Science Foundation grants EB00303, CBET1066661This abstract is from the Experimental Biology 2019 Meeting. There is no full text article associated with this abstract published in The FASEB Journal .