Premium
A thermogenic‐like brown adipose tissue phenotype is dispensable for enhanced glucose tolerance in female mice
Author(s) -
Winn Nathan,
AcinPerez Rebeca,
Woodford Makenzie,
Hansen Sarah,
Haney Megan,
Ayedun Lolade,
Rector R. Scott,
VieiraPotter Victoria,
Shirihai Orian,
Sacks Harold,
Kanaley Jill,
Padilla Jaume
Publication year - 2019
Publication title -
the faseb journal
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.709
H-Index - 277
eISSN - 1530-6860
pISSN - 0892-6638
DOI - 10.1096/fasebj.2019.33.1_supplement.lb564
Subject(s) - brown adipose tissue , thermogenesis , phenotype , biology , endocrinology , medicine , white adipose tissue , adipose tissue , glucose homeostasis , obesity , genetics , insulin resistance , gene
The prevailing dogma is that thermogenic brown adipose tissue (BAT) contributes to improvements in glucose homeostasis in obesogenic animal models; though, much of the evidence supporting this premise is from thermostressed rodents. Whether modulation of the BAT morphology/function drives changes in glucoregulation at thermoneutrality requires further investigation. We utilized loss and gain‐of‐function approaches including genetic manipulation of the lipolytic enzyme Pnpla2 , change in environmental temperature, and lifestyle interventions to comprehensively test the premise that a thermogenic‐like BAT phenotype is coupled with enhanced glucose tolerance in female mice. In contrast to this hypothesis, we found that: (i) compared to mice kept at thermoneutrality, enhanced activation of BAT and its thermogenic phenotype via chronic mild cold stress does not improve glucose tolerance in obese mice; (ii) silencing of the Pnpla2 in interscapular BAT causes a brown‐to‐white phenotypic shift but does not disturb glucose tolerance in lean mice; and (iii) exercise and low‐fat diet improve glucose tolerance in obese mice but these effects do not track with a thermogenic BAT phenotype. Collectively, these findings indicate that a thermogenic‐like BAT phenotype is not linked to heightened glucose tolerance in female mice. This abstract is from the Experimental Biology 2019 Meeting. There is no full text article associated with this abstract published in The FASEB Journal .