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MicroRNA (miR‐93) Overexpression Selectively in Adipose Tissue Induces Obesity
Author(s) -
Chen Elizabeth,
Wu Hsiao Li,
Chen Yen Hao
Publication year - 2019
Publication title -
the faseb journal
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.709
H-Index - 277
eISSN - 1530-6860
pISSN - 0892-6638
DOI - 10.1096/fasebj.2019.33.1_supplement.lb563
Subject(s) - adipose tissue , endocrinology , medicine , microrna , obesity , overweight , type 2 diabetes , polycystic ovary , weight loss , weight gain , body weight , biology , diabetes mellitus , insulin resistance , gene , genetics
For years, many women have complained of gaining weight faster than others even when consuming the same type and amount of food. By working in the lab, we may have found the reasons for these complaints. There is a higher statistic of women with polycystic ovary syndrome (PCOS) that are overweight or obese, causing them to be at a higher risk of diabetes and cardiovascular disease. In our previous studies, we found that the expression of microRNA‐93 (miR‐93) was up‐regulated in adipose tissue from PCOS women. As a result, we generated an adipose‐selective miR‐93 over‐expression mouse (miR‐93AT+ mice) model by using Cre/loxP DNA recombination. The expression of miR‐93 was specifically increased in adipose tissue but not in other tissues in these miR‐93AT+ mice. The weight of the female miR‐93AT+ mice were significantly higher than the wild type mice (miR‐93AT+ vs wild type = 25.8 ± 1.0 (n=7) vs 22.4 ± 1.1 (n=5), Mean ± SE) in a normal diet. Additionally, the female miR‐93AT+ mice gained weight faster (increased approximately 51% of weight) than the wild type mice (increased approximately 29% of weight) when food was switched to the high‐fat diet (HFD) for 40 days. These data suggest that miR‐93 specific over‐expression in the adipose tissue may answer the question of why some women gain weight easier than others. Additional studies are in progress to understand the mechanisms under the miR‐93 over‐expression and obesity.Percentage weight gain of day 0 during the high‐fat diet.This abstract is from the Experimental Biology 2019 Meeting. There is no full text article associated with this abstract published in The FASEB Journal .