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Evaluation of Alpha‐glucosidase Inhibition by Alcoholic Extract of Garcinia Kola
Author(s) -
Obih Patience O,
Obih JohnClifford A,
Nwogu Ivy
Publication year - 2019
Publication title -
the faseb journal
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.709
H-Index - 277
eISSN - 1530-6860
pISSN - 0892-6638
DOI - 10.1096/fasebj.2019.33.1_supplement.lb45
Subject(s) - garcinia kola , acarbose , traditional medicine , diabetes mellitus , medicine , alpha glucosidase , garcinia , chemistry , biochemistry , enzyme , endocrinology
Diabetes mellitus is a serious chronic disorder and the 7th leading cause of death in the United States of America. It is a metabolic disorder where the body cannot properly metabolize food for energy. It is a chronic condition that is dramatically increasing every year. Recently, more individuals with diabetes are interested in alternative strategies for treating diabetes such as herbal remedies. The objective of this study was to investigate the alpha‐glucosidase inhibitory potential of alcoholic extracts of Garcinia Kola (Bitter Kola) in regulating post‐prandial glucose levels in diabetes. Garcinia Kola , known in Africa as “Wonder plant” was selected for this study because of its numerous use cited in literqture. The in vitro assay was carried out in a 96‐well microplate. The reaction mixture consisted of 2.5 μL of various dilutions of Garcinia extract, 122.5μl of 0.2M phosphate buffer and 62.5 μL of 5mM para‐nitrophenyl‐α‐D‐glucopyranoside. The mixture was pre‐incubated at 37 °C for 5 min followed by the addition of 62.5 μL of α‐glucosidase solution. The mixture was then incubated for an additional 10 minutes and analyzed with a ThermoScientific® Multiskan Spectrophometer at an absorbance of 400nm. Acarbose was used as a positive control of α‐glucosidase inhibitor. From our observation, Garcinia Kola produced inhibition of alpha‐glucosidase, an indication that it may be useful in reducing hyperglycemia. Support or Funding Information Center of Excellence/BUILD, Xavier University of Louisiana This abstract is from the Experimental Biology 2019 Meeting. There is no full text article associated with this abstract published in The FASEB Journal .