Premium
High‐precision and scalable fabrication of biomimetic culture environments for enhancing adherent mammalian cell and tissue engineering
Author(s) -
Ghazizadeh Hamed,
Fisher Elliot,
Gray Kevin M,
Geisse Nicholas A
Publication year - 2019
Publication title -
the faseb journal
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.709
H-Index - 277
eISSN - 1530-6860
pISSN - 0892-6638
DOI - 10.1096/fasebj.2019.33.1_supplement.lb430
Subject(s) - in vivo , nanotechnology , tissue engineering , mesenchymal stem cell , cell , cell culture , microbiology and biotechnology , materials science , chemistry , biophysics , biology , computational biology , biochemistry , genetics
Cells maintained in vitro typically lose various structural and functional phenotypes that are found in vivo. Generation of biomimetic substrates typically involves costly or hard‐to‐reproduce techniques that are often incompatible with many standard assays. Here, we will present a novel method of generating surfaces that mimic the nanoscale shape and structure of the collagen ECM. The fabrication scheme described is highly reproducible, scalable, and amenable to integration with most end‐point assays, including high‐NA optical microscopy. Further, we present techniques to can fabricate biomimetic culture surfaces out of elastomers that can be stretched in order to reproduce mechanical stimuli that are critical in the development and function of some tissues. Various cell types were tested and were amenable to this approach. Cardiac tissues showed more in vivo‐like myofibril alignment, sarcomere spacing and width, and expression of CM‐specific proteins. Stem‐cell derived CMs similarly showed earlier onset of maturity and differentiation. We will show that the nanoscale patterning also maintains the migratory phenotype of metastatic cancer cells in vitro. Finally, we will demonstrate that this patterning approach can be extended to high‐throughput electrophysiological assays when combined with micro‐electrode arrays (MEAs) without compromising signal fidelity but enhancing sensitivities to drugs of known in vivo effect. Support or Funding Information NIH SBIR Funding This abstract is from the Experimental Biology 2019 Meeting. There is no full text article associated with this abstract published in The FASEB Journal .