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Effect of bile acids feeding on atherosclerosis in ApoE −/− mice
Author(s) -
Escher Geneviève,
Zurkinden Line,
Vogt Bruno
Publication year - 2019
Publication title -
the faseb journal
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.709
H-Index - 277
eISSN - 1530-6860
pISSN - 0892-6638
DOI - 10.1096/fasebj.2019.33.1_supplement.lb402
Subject(s) - cholesterol , cholic acid , chenodeoxycholic acid , medicine , endocrinology , bile acid , apolipoprotein e , chemistry , reverse cholesterol transport , absorption (acoustics) , lipoprotein , biology , materials science , disease , composite material
Bile acids (BAs) account for lipids absorption in the small intestine and are key regulators of cholesterol homeostasis as they clear cholesterol excess in the liver. Therefore, we hypothesize that long term BAs feeding could prevent the development of atherosclerosis. To prove this, we took ApoE −/− mice and fed them for 8 weeks with a western diet (WD) or a WD containing 0.1% cholic acid (CA) (WD‐CA) or 0.1% chenodeoxycholic acid (CDCA) (WD‐CDCA) and assessed their plasma lipoprotein and hepatic lipid content, intestinal cholesterol absorption and atherosclerosis. WD‐CA increased plasma LDL‐C by ~75%, HDL‐C by ~30%, hepatic cholesterol by ~40% and cholesterol ester by ~300%. WD‐CA additionally increased cholesterol absorption by 25%. All these changes led to a 100% increase of the atherosclerotic lesions. In contrary, WD‐CDCA had no effect on plasma lipoproteins and hepatic cholesterol but massively increased hepatic cholesterol ester. WD‐CDCA additionally decreased cholesterol absorption by 25%, but atherosclerotic lesions were of the same magnitude as in ApoE −/− mice fed with WD. Long term administration of CA and CDCA enhanced cholesterol accumulation in the liver and had no benefit towards atherosclerosis development in ApoE −/− mice. Even more, CA was atherogenic, mainly because of increased plasma lipids and intestinal cholesterol absorption. Despite improved intestinal cholesterol elimination, CDCA treatment did not reduce atherosclerosis. Other strategies are required to reduce atherosclerosis without causing liver damage in mice. Support or Funding Information None This abstract is from the Experimental Biology 2019 Meeting. There is no full text article associated with this abstract published in The FASEB Journal .