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Assessment of Cellular Respiration and Oxidative Phosphorylation in Cocks Semen after Freezing
Author(s) -
Nikitkina Elena,
Musidray Artem,
Timofeeva Svetlana,
Krutikova Anna,
Plemyashov Kirill,
Pleshanov Nikolay
Publication year - 2019
Publication title -
the faseb journal
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.709
H-Index - 277
eISSN - 1530-6860
pISSN - 0892-6638
DOI - 10.1096/fasebj.2019.33.1_supplement.lb307
Subject(s) - oxidative phosphorylation , respiration , adenosine triphosphate , cellular respiration , chemistry , phosphorylation , atp synthase , mitochondrion , sperm , respiration rate , biochemistry , glycolysis , adenosine diphosphate , adenine nucleotide , semen , metabolism , biology , enzyme , anatomy , platelet , nucleotide , immunology , botany , gene , platelet aggregation
The biochemical processes associated with the generation of energy (ATP) ‐ oxidative phosphorylation and glycolysis ‐ occupy a central place in the vital activity of cells. Numerous studies have established that the ratio of respiration and phosphorylation is a sensitive indicator of the functional state of the cell and the whole organism. Oxidative phosphorylation (OXPHOS) is the metabolic pathway in which cells use enzymes to oxidize nutrients, thereby releasing energy which is used to produce adenosine triphosphate (ATP). OXPHOS is the terminal process of cellular respiration (CR). Aim of the study was to evaluate the ratio of respiration and phosphorylation in cocks sperm after freezing. Semen from 10 cocks was frozen. CR was assessed by the polarographic method. OXPHOS can be evaluated by the reaction CR on adding 2,4‐dinitrophenol (2,4 ‐ DNP ) to semen sample. 2,4 ‐ DNP disrupt the proton gradient by carrying protons across a membrane and uncouples proton pumping from ATP synthesis because it carries protons across the inner mitochondrial membrane. As a result it increases respiration rate. If the respiration rate increases – there is good OXPHOS. Results are depicted as ranges, percentages of totals and averages ± SEs. The addition of 2,4 ‐ DNP to sperm samples caused a significant increase in CR (1.8 ± 0.40 times). This suggests that the CR of freshly diluted cocks sperm is highly associated with phosphorylation. We observed a drop in respiratory stimulation by 2,4 ‐ DNP (1,7±0,11 times) after thawing. This is an indication of an increase in free oxidation not associated with oxidative phosphorylation. The CR stimulation by 2,4‐DNP in the frozen samples ranged from 1.4 to 2.3, and the fertility of eggs ranged from 24 to 95%. The correlation between egg fertilization and stimulation of respiration by 2,4‐DNP was high (r = 0,76, P <0,01). Assessment of oxidative phosphorylation can serve as a marker of sperm viability after freezing and as criterion for predicting the fertilizing ability. Support or Funding Information Authors acknowledge financial support from Russian Science Foundation, Grant No: 18‐16‐00071 This abstract is from the Experimental Biology 2019 Meeting. There is no full text article associated with this abstract published in The FASEB Journal .