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Microbial Co‐occurrence Patterns and Keystone Species in the Gut Microbial Community of Mice in Response to Stress and Chondroitin Sulfate Disaccharide
Author(s) -
Li Robert W.,
Liu Fang,
Li Xhaojie,
Xue Changhu,
Tang Qingjuan
Publication year - 2019
Publication title -
the faseb journal
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.709
H-Index - 277
eISSN - 1530-6860
pISSN - 0892-6638
DOI - 10.1096/fasebj.2019.33.1_supplement.lb300
Subject(s) - proteobacteria , metaproteomics , microbial population biology , firmicutes , biology , metagenomics , gut flora , microbiome , bacteroidetes , microbial ecology , microbiology and biotechnology , ecology , biochemistry , bacteria , bioinformatics , genetics , gene , 16s ribosomal rna
Detecting microbial interactions is essential to the understanding of the structure and function of the gut microbiome. In this study, microbial co‐occurrence patterns were inferred using a random matrix theory based approach in the gut microbiome of mice in response to chondroitin sulfate disaccharide (CSD) under healthy and stressed conditions. While the overall size and structure of the global networks were similar, the network composition was distinctly different among various experimental conditions. The exercise stress disrupted the network composition and microbial co‐occurrence patterns. 34 Operational Taxonomic Units (OTU) were identified as module hubs and connectors, likely acting as generalists in the microbial community. Mucispirillum schaedleri acted as a connector in the stressed network in response to CSD supplement and may play a key role for bridging intimate interactions between the host and its microbiome. Several modules strongly correlated with physiological parameters were detected. For example, Modules M02 (under stress) and S05 (stress + CSD) were strongly correlated with blood urea nitrogen levels ( r = 0.90 and − 0.75, respectively). A positive correlation between node connectivity of the OTUs assigned to Proteobacteria with superoxide dismutase activities under stress ( r = 0.57, P <0.05) provided further evidence that Proteobacteria can be developed as a potential pathological marker. Moreover, the results suggest that altered network topologies may have important functional implications. Our findings provided novel insights into microbial interactions in gut microbial communities of the mice in response to stress and CSD supplement and may facilitate future endeavor in microbial community engineering. Support or Funding Information National Natural Science Foundation of China (No. U1606403 to CX) This abstract is from the Experimental Biology 2019 Meeting. There is no full text article associated with this abstract published in The FASEB Journal .