Premium
Immune response mediated by NRLP3 expression in children infected with Respiratory Syncytial Virus
Author(s) -
NETO ERASMO ASSUMPCAO,
DE ANGELIS KATIA,
DE PAULA VIEIRA RODOLFO,
CONSOLIMCOLOMBO FERNANDA MARCIANO
Publication year - 2019
Publication title -
the faseb journal
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.709
H-Index - 277
eISSN - 1530-6860
pISSN - 0892-6638
DOI - 10.1096/fasebj.2019.33.1_supplement.lb280
Subject(s) - gene expression , virus , immune system , rna , respiratory system , gene , complementary dna , real time polymerase chain reaction , biology , paramyxoviridae , medicine , virology , microbiology and biotechnology , immunology , viral disease , biochemistry
OBJECTIVE To evaluate the expression of the NRLP3 gene present in the inflammatory pathway and its regulation against pediatric infection with Respiratory Syncytial Virus (RSV) METHODS : 31 patients younger than 2 years of age were divided into RSV (n = 11) and control (n = 20) groups. Five cc of venous blood was collected in EDTA tube. The tubes were centrifuged and, after separation of the blood constituents, the buff coat was added and RNA latter Total RNA was isolated. The amount and quality (280/260) of RNA were assessed by reading spectrophotometry at Nanodrop Total sample RNA was retranscribed and the cDNA/reaction was used for relative expression analysis of the NLRP3 inflammation gene by means of gene‐specific type‐specific fluorogenic probes and qPCR. The ExpressionSuite software in the Cloud Platform was used to obtain the raw data (Ct). The GAPDH gene was used as endogenous control for raw data normalization (Ct). The gene expression data were analyzed using the comparative method of Ct RESULTS The analysis of the data obtained in the qPCR technique for the expression of the NRLP3 gene was that in the patients infected with Respiratory Syncytial Virus it has a lower expression of it in relation to the control patients. Statistical analysis was performed using GraphPad Prism® software, using t test. presenting (P <0.05) between the groups compared. CONCLUSION With the present analysis of the studied study we can observe a lower expression of NRLP3 in pediatric patients infected with RSV in relation to the control patients without infection, within this result we can collaborate with the literature in the description of the behavior of the NRLP3 gene that is not yet well defined as in some articles demonstrate in the bronchial epithelial cells, that when the infection is expected an increase in the expression of the gene and consequently of its products the pro‐inflammatory cytokines and the caspases for induction to cell death. However in our study we have seen that the decrease in NRLP3 expression in bloodstream cells may demonstrate a mechanism of escape of the virus so that the cell does not produce pro‐inflammatory cytokines and caspases for induction of cell death so that it can maintain alive and replicating for longer. Support or Funding Information FAPESPFold change of expression mRNAThis abstract is from the Experimental Biology 2019 Meeting. There is no full text article associated with this abstract published in The FASEB Journal .