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Does DMD‐10 independently affect levels of GLR‐1?
Author(s) -
Nammour Josette,
McGehee Annette
Publication year - 2019
Publication title -
the faseb journal
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.709
H-Index - 277
eISSN - 1530-6860
pISSN - 0892-6638
DOI - 10.1096/fasebj.2019.33.1_supplement.lb269
Subject(s) - mcherry , mutant , caenorhabditis elegans , wild type , biology , hek 293 cells , microinjection , microbiology and biotechnology , genetics , green fluorescent protein , gene
We aim to study the mechanisms that affect the Caenorhabditis elegans glutamate receptor GLR‐1. The roles of genes that may be involved in the regulation of GLR‐1 levels are assessed using GLR‐1‐dependent behavioral tests such as spontaneous reversals assays and nose touch assays. Results from our laboratory have shown that dmd‐10 mutants exhibit a low rate of spontaneous reversals when compared to the wildtype strain. We generated rescue strains by cloning DMD‐10 and microinjecting it into dmd‐10 mutant worms to assess the effect of wildtype DMD‐10. The constructs developed for microinjection contained the dmd‐10 gene being driven by either the glr‐1 promoter (Pglr‐1::dmd‐10) or its own promoter (Pdmd‐10::dmd‐10). Pglr‐1 rescue strains, which consist of the dmd‐10 mutant and wildtype DMD‐10 expressed in cells that express GLR‐1, were compared to the dmd‐10 mutant strain and wildtype using behavioral assays. Pglr‐1 rescue strains did not exhibit behavioral differences from dmd‐10 mutant worms. Pdmd‐10 rescue strains have been generated and are currently being tested for behavioral rescue. Additionally, we are developing constructs that will allow us to visualize the location of dmd‐10 neurons in the wildtype worms using the red fluorescent protein, mCherry. This abstract is from the Experimental Biology 2019 Meeting. There is no full text article associated with this abstract published in The FASEB Journal .