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Effects of Photobiomodulation on Apoptosis Process After Induced Myocardial Infarction in Rats: Analysis of Gene Expression
Author(s) -
Maretti Joao Victor,
Manchini Martha Trindade,
Silva Jose Antonio,
Serra Andrey Jorge,
Feliciano Regiane Santos
Publication year - 2019
Publication title -
the faseb journal
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.709
H-Index - 277
eISSN - 1530-6860
pISSN - 0892-6638
DOI - 10.1096/fasebj.2019.33.1_supplement.lb267
Subject(s) - medicine , ventricle , myocardial infarction , cardiology , apoptosis , muscle hypertrophy , fibrosis , cardioprotection , infarction , contraction (grammar) , biology , biochemistry
Cardiovascular Diseases (CVD) are the main cause of death in Brazil and in the rest of the world. Ischemic heart diseases represent 30% of deaths by CVD in occidental countries. It is well known that Myocardial Infarction (MI) harms contraction and is responsible of a hypertrophy process of the cardiomyocytes, besides the reduction of ventricle wall thickness, modifying extracellular matrix and promoting metabolic changes. Those metabolic processes stimulate a compensatory rearrangement by targeting three different morphologic regions: infarcted area, borderline area and the remote area to the MI. New treatments are aimed at the modulation of various cardiac processes, such as cardiac muscle contraction, metabolism, apoptosis, fibrosis formation and control of hypertrophy, with the objective of revert or prevent damage on those heart areas. Nowadays, Low‐Level Laser Therapy (LLLT) has become an alternative treatment by its capacity of modulation various biological processes and, depending on dose and wavelength parameters, anti‐inflammatory proprieties and faster cell proliferation. However, the action of the LLLT has not yet been elucidated and little is known about the remaining myocardium behaviour on this treatment, specially when related to apoptosis. Thus, the present study aims the evaluation of LLLT effects on genes related to apoptosis, by analysing TP53, MAP3K2, Bax, MAPK14 and Fas mRNA. The study, approved by the local ethics committee, was designed by the promotion of anterior descending coronary artery occlusion and posterior laser irradiation in the remote region to the myocardial infarction in rats, which were submitted to an echocardiography to evaluate myocardial function after therapeutics. Heart samples underwent an analysis by Real‐Time Polymerase Chain Reaction Array. Data showed that LLLT was capable of modulating apoptosis process, by downregulating TP53 gene, when compared to rats which underwent only anterior descending coronary artery occlusion. The study concludes that LLLT may stimulate cellular survival after MI, by the inhibition of apoptosis of remote region cells. Support or Funding Information CNPq, CAPES, UNINOVE This abstract is from the Experimental Biology 2019 Meeting. There is no full text article associated with this abstract published in The FASEB Journal .